Immobilization of DNA for Scanning Probe Microscopy

Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demons...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-11, Vol.89 (21), p.10129-10133
Main Authors: Allison, D. P., Bottomley, L. A., Thundat, T., Brown, G. M., Woychik, R. P., Schrick, J. J., Jacobson, K. Bruce, Warmack, R. J.
Format: Article
Language:English
Subjects:
Acetates
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA probes
DNA, Bacterial - ultrastructure
DNA, Circular - ultrastructure
Drug Stability
Escherichia coli
Fundamental and applied biological sciences. Psychology
Image contrast
Imaging
Indicators and Reagents
Medical research
Mica
Microscopes
Microscopy - methods
Microscopy, Scanning Tunneling - methods
Molecular probes
Molecules
Phosphorus Radioisotopes
Plasmids
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Description
Summary:Reproducible scanning tunneling microscope and atomic force microscope images of entire molecules of uncoated plasmid DNA chemically bound to surfaces are presented. The chemically mediated immobilization of DNA to surfaces and subsequent scanning tunneling microscope imaging of DNA molecules demonstrate that the problem of molecular instability to forces exerted by the probe tip, inherent with scanning probe microscopes, can be prevented.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.21.10129