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Photorepair mutants of Arabidopsis
UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dim...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1997-07, Vol.94 (14), p.7441-7445 |
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| container_end_page | 7445 |
| container_issue | 14 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 94 |
| creator | Jiang, C.Z. (University of California, Davis, CA.) Yee, J Mitchell, D.L Britt, A.B |
| description | UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dimer, Arabidopsis thaliana, Drosophila melanogaster, Crotalus atrox, and Xenopus laevis have recently been shown to photoreactivate both CPDs and 6-4 products. We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system |
| doi_str_mv | 10.1073/pnas.94.14.7441 |
| format | article |
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(University of California, Davis, CA.) ; Yee, J ; Mitchell, D.L ; Britt, A.B</creator><creatorcontrib>Jiang, C.Z. (University of California, Davis, CA.) ; Yee, J ; Mitchell, D.L ; Britt, A.B</creatorcontrib><description>UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dimer, Arabidopsis thaliana, Drosophila melanogaster, Crotalus atrox, and Xenopus laevis have recently been shown to photoreactivate both CPDs and 6-4 products. We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.94.14.7441</identifier><identifier>PMID: 9750104</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>ADN ; Arabidopsis - genetics ; ARABIDOPSIS THALIANA ; Biological Sciences ; Botany ; Carbon-Carbon Lyases - metabolism ; Cephalopelvic disproportion ; COMPLEMENTATION ; Crotalus atrox ; CYCLOBUTANE PYRIMIDINE DIMERS ; CYCLOBUTANE PYRIMIDINE PHOTOLYASE ; Deoxyribodipyrimidine Photo-Lyase - genetics ; Deoxyribodipyrimidine Photo-Lyase - metabolism ; Dimers ; DNA ; DNA damage ; DNA MODIFICATION ; DNA REPAIR ; Drosophila melanogaster ; Escherichia coli ; Flowers & plants ; Genetic mutation ; GENETICA ; GENETICS ; GENETIQUE ; Genomes ; Lesions ; LIASAS ; LYASE ; LYASES ; MUTACION ; MUTANT ; MUTANTES ; MUTANTS ; MUTATION ; PHOTOREACTIVATION ; Pyrimidine dimers ; Pyrimidine Dimers - metabolism ; PYRIMIDINE PYRIMIDINONE DIMERS ; RADIACION ULTRAVIOLETA ; RAYONNEMENT ULTRAVIOLET ; Root growth ; Saccharomyces cerevisiae ; Seedlings ; ULTRAVIOLET RADIATION ; Ultraviolet Rays ; Xenopus laevis</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1997-07, Vol.94 (14), p.7441-7445</ispartof><rights>Copyright 1997 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jul 8, 1997</rights><rights>Copyright © 1997, The National Academy of Sciences of the USA 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c606t-91d36285c8c75911144390f23971c22b0e51658211753a9c5efad34e682f57933</citedby><cites>FETCH-LOGICAL-c606t-91d36285c8c75911144390f23971c22b0e51658211753a9c5efad34e682f57933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/94/14.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42697$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42697$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9750104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, C.Z. (University of California, Davis, CA.)</creatorcontrib><creatorcontrib>Yee, J</creatorcontrib><creatorcontrib>Mitchell, D.L</creatorcontrib><creatorcontrib>Britt, A.B</creatorcontrib><title>Photorepair mutants of Arabidopsis</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>UV radiation induces two major DNA damage products, the cyclobutane pyrimidine dimer (CPD) and, at a lower frequency, the pyrimidine (6-4) pyrimidinone dimer (6-4 product). Although Escherichia coli and Saccharomyces cerevisiae produce a CPD-specific photolyase that eliminates only this class of dimer, Arabidopsis thaliana, Drosophila melanogaster, Crotalus atrox, and Xenopus laevis have recently been shown to photoreactivate both CPDs and 6-4 products. We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system</description><subject>ADN</subject><subject>Arabidopsis - genetics</subject><subject>ARABIDOPSIS THALIANA</subject><subject>Biological Sciences</subject><subject>Botany</subject><subject>Carbon-Carbon Lyases - metabolism</subject><subject>Cephalopelvic disproportion</subject><subject>COMPLEMENTATION</subject><subject>Crotalus atrox</subject><subject>CYCLOBUTANE PYRIMIDINE DIMERS</subject><subject>CYCLOBUTANE PYRIMIDINE PHOTOLYASE</subject><subject>Deoxyribodipyrimidine Photo-Lyase - genetics</subject><subject>Deoxyribodipyrimidine Photo-Lyase - metabolism</subject><subject>Dimers</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA MODIFICATION</subject><subject>DNA REPAIR</subject><subject>Drosophila melanogaster</subject><subject>Escherichia coli</subject><subject>Flowers & plants</subject><subject>Genetic mutation</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>Genomes</subject><subject>Lesions</subject><subject>LIASAS</subject><subject>LYASE</subject><subject>LYASES</subject><subject>MUTACION</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>MUTATION</subject><subject>PHOTOREACTIVATION</subject><subject>Pyrimidine dimers</subject><subject>Pyrimidine Dimers - metabolism</subject><subject>PYRIMIDINE PYRIMIDINONE DIMERS</subject><subject>RADIACION ULTRAVIOLETA</subject><subject>RAYONNEMENT ULTRAVIOLET</subject><subject>Root growth</subject><subject>Saccharomyces cerevisiae</subject><subject>Seedlings</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><subject>Xenopus laevis</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFEEQhhtR4iZ6FgRlyUFPs6nq7wIvIfgFAQXNuemd7UlmmZ0eu2dE_3162WX8OOipD-_zFtX1MPYMYYVgxMXQ-7wiuUK5MlLiA7ZAIKy0JHjIFgDcVFZy-Zid5rwFAFIWTtgJGQUIcsHOP9_FMaYw-DYtd9Po-zEvY7O8TH7dbuKQ2_yEPWp8l8PT43vGbt69_Xr1obr-9P7j1eV1VWvQY0W4EZpbVdvaKEJEKQVBwwUZrDlfQ1ColeWIRglPtQqN3wgZtOWNMiTEGXtzmDtM613Y1KEfk-_ckNqdTz9d9K37M-nbO3cbvzsurIRSf3Wsp_htCnl0uzbXoet8H-KUnSEtrCL7XxB1uRoiL-D5X-A2TqkvN3AcUGijNRbo4gDVKeacQjMvjOD2itxekSPpULq9otJ48fs_Z_7opOSvj_m-OKfzANdMXTeGH2MhX_6TLMDzA7DNRfJMSK7J_Nqj8dH529Rmd_MFiQwoVFyJe9NBtBI</recordid><startdate>19970708</startdate><enddate>19970708</enddate><creator>Jiang, C.Z. (University of California, Davis, CA.)</creator><creator>Yee, J</creator><creator>Mitchell, D.L</creator><creator>Britt, A.B</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences of the USA</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970708</creationdate><title>Photorepair mutants of Arabidopsis</title><author>Jiang, C.Z. (University of California, Davis, CA.) ; Yee, J ; Mitchell, D.L ; Britt, A.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c606t-91d36285c8c75911144390f23971c22b0e51658211753a9c5efad34e682f57933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ADN</topic><topic>Arabidopsis - genetics</topic><topic>ARABIDOPSIS THALIANA</topic><topic>Biological Sciences</topic><topic>Botany</topic><topic>Carbon-Carbon Lyases - metabolism</topic><topic>Cephalopelvic disproportion</topic><topic>COMPLEMENTATION</topic><topic>Crotalus atrox</topic><topic>CYCLOBUTANE PYRIMIDINE DIMERS</topic><topic>CYCLOBUTANE PYRIMIDINE PHOTOLYASE</topic><topic>Deoxyribodipyrimidine Photo-Lyase - genetics</topic><topic>Deoxyribodipyrimidine Photo-Lyase - metabolism</topic><topic>Dimers</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA MODIFICATION</topic><topic>DNA REPAIR</topic><topic>Drosophila melanogaster</topic><topic>Escherichia coli</topic><topic>Flowers & plants</topic><topic>Genetic mutation</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>Genomes</topic><topic>Lesions</topic><topic>LIASAS</topic><topic>LYASE</topic><topic>LYASES</topic><topic>MUTACION</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>MUTATION</topic><topic>PHOTOREACTIVATION</topic><topic>Pyrimidine dimers</topic><topic>Pyrimidine Dimers - metabolism</topic><topic>PYRIMIDINE PYRIMIDINONE DIMERS</topic><topic>RADIACION ULTRAVIOLETA</topic><topic>RAYONNEMENT ULTRAVIOLET</topic><topic>Root growth</topic><topic>Saccharomyces cerevisiae</topic><topic>Seedlings</topic><topic>ULTRAVIOLET RADIATION</topic><topic>Ultraviolet Rays</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, C.Z. 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We describe the isolation and characterization of two new classes of mutants of Arabidopsis, termed uvr2 and uvr3, that are defective in the photoreactivation of CPDs and 6-4 products, respectively. We demonstrate that the CPD photolyase mutation is genetically linked to a DNA sequence encoding a type II (metazoan) CPD photolyase. In addition, we are able to generate plants in which only CPDs or 6-4 products are photoreactivated in the nuclear genome by exposing these mutants to UV light and then allowing them to repair one or the other class of dimers. This provides us with a unique opportunity to study the biological consequences of each of these two major UV-induced photoproducts in an intact living system</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>9750104</pmid><doi>10.1073/pnas.94.14.7441</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1997-07, Vol.94 (14), p.7441-7445 |
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| subjects | ADN Arabidopsis - genetics ARABIDOPSIS THALIANA Biological Sciences Botany Carbon-Carbon Lyases - metabolism Cephalopelvic disproportion COMPLEMENTATION Crotalus atrox CYCLOBUTANE PYRIMIDINE DIMERS CYCLOBUTANE PYRIMIDINE PHOTOLYASE Deoxyribodipyrimidine Photo-Lyase - genetics Deoxyribodipyrimidine Photo-Lyase - metabolism Dimers DNA DNA damage DNA MODIFICATION DNA REPAIR Drosophila melanogaster Escherichia coli Flowers & plants Genetic mutation GENETICA GENETICS GENETIQUE Genomes Lesions LIASAS LYASE LYASES MUTACION MUTANT MUTANTES MUTANTS MUTATION PHOTOREACTIVATION Pyrimidine dimers Pyrimidine Dimers - metabolism PYRIMIDINE PYRIMIDINONE DIMERS RADIACION ULTRAVIOLETA RAYONNEMENT ULTRAVIOLET Root growth Saccharomyces cerevisiae Seedlings ULTRAVIOLET RADIATION Ultraviolet Rays Xenopus laevis |
| title | Photorepair mutants of Arabidopsis |
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