Gender-Specific Frequency of Background Somatic Mutations at the Hypoxanthine Phosphoribosyltransferase Locus in Cord Blood T Lymphocytes from Preterm Newborns

Limited information is available regarding the frequency, spectrum, and clinical relevance of somatic mutations in the developing fetus. The goal of this study was to determine somatic mutant frequencies (Mfs) at the hypoxanthine phosphoribosyltransferase (HPRT) reporter gene in cord blood T lymphoc...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1999-01, Vol.96 (2), p.586-591
Main Authors: Yoshioka, Makoto, Vacek, Pamela M., Poseno, Tina, Silver, Robert, Finette, Barry A.
Format: Article
Language:English
Subjects:
Biological Sciences
Blood
Children
Cigarette smoking
Cigarettes
Cord blood
Female
Fetal Blood - enzymology
Fetus - metabolism
Fetuses
Genetics
Gestational Age
Humans
Hypoxanthine Phosphoribosyltransferase - genetics
Infant, Newborn
Infants
Male
Mutation
Mutation - genetics
Nicotiana - genetics
Obstetric Labor, Premature - genetics
Plants, Toxic
Pregnancy
Regression Analysis
Reproduction
Sex
Sexes
Smoking - genetics
Somatic mutation
T lymphocytes
T-Lymphocytes - enzymology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Limited information is available regarding the frequency, spectrum, and clinical relevance of somatic mutations in the developing fetus. The goal of this study was to determine somatic mutant frequencies (Mfs) at the hypoxanthine phosphoribosyltransferase (HPRT) reporter gene in cord blood T lymphocytes from preterm infants to gain insight into in utero mutational events. Mf determinations were made by using the HPRT T cell cloning assay on cord blood samples from 52 preterm infants. Natural logarithm Mfs (InMfs) from preterm infants were compared with results from our database for full-term infants. Our analysis revealed higher InMfs in cord blood T lymphocytes from preterm compared with full-term infants (P = 0.008). In addition, preterm females had significantly higher InMfs compared with full-term females (P < 0.001), whereas preterm males were found to have significantly lower InMfs than preterm females (P = 0.005). Regression analyses also demonstrate a significant relationship between InMf and gestational age for preterm females that does not exist for preterm males. These results demonstrate the gender-specific association between Mf and age in humans.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.96.2.586