In vitro glycoxidation of insoluble fibrous type I collagen: solubilization and advanced glycation end products

The deleterious effects of glycoxidation are dependent on the half-life of proteins. Collagen, the main component of extracellular matrices, is a long live protein and thus may be sensitive to the glycoxidation process. We incubated calf skin fibrous type I collagen in PBS at 37 degrees C with gluco...

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Bibliographic Details
Published in:Journal of Protein Chemistry 2003-08, Vol.22 (6), p.527-531
Main Authors: Meli, M, Granouillet, R, Reynaud, E, Chamson, A, Frey, J, Perier, C
Format: Article
Language:English
Subjects:
Advanced glycosylation end products
Amidines - pharmacology
Animals
Arginine - analogs & derivatives
Arginine - analysis
Cattle
Collagen
Collagen (type I)
Collagen Type I - chemistry
Collagen Type I - metabolism
Contamination
Free radicals
Glycation End Products, Advanced - analysis
Glycosylation
Lysine - analogs & derivatives
Lysine - analysis
Molecular biology
Oxidation
Proteins
Proteolysis
Solubility
Solubilization
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Summary:The deleterious effects of glycoxidation are dependent on the half-life of proteins. Collagen, the main component of extracellular matrices, is a long live protein and thus may be sensitive to the glycoxidation process. We incubated calf skin fibrous type I collagen in PBS at 37 degrees C with glucose. The fibrous type I collagen was solubilized and an increase in the amount of advanced glycation end products of the solubilized fraction was observed. As there was no bacterial contamination and no proteolytic activities in the incubation medium, the solubilization of fibrous type I collagen is probably due to the speculative production of the free radicals in our experimental conditions. To test this hypothesis, fibrous type I collagen was incubated in PBS with AAPH (2,2'azo-bis 2-aminodinopropane) a free radicals generator. AAPH induced a dramatic and dose dependent solubilization of fibrous type I collagen.
ISSN:0277-8033
1572-3887
1573-4943
DOI:10.1023/B:JOPC.0000005502.48925.34