A New Pathway of Staphylococcal Pathogenesis: Apoptosis-Like Death Induced by Staphopain B in Human Neutrophils and Monocytes

Circulating neutrophils and monocytes form the first line of cellular defense against invading bacteria. Here, we describe a novel and specific mechanism of disabling and eliminating phagocytes by Staphylococcus aureus. Staphopain B (SspB) selectively cleaved CD11b on phagocytes, which rapidly acqui...

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Bibliographic Details
Published in:Journal of innate immunity 2009-01, Vol.1 (2), p.98-108
Main Authors: Smagur, Jan, Guzik, Krzysztof, Magiera, Lukasz, Bzowska, Malgorzata, Gruca, Milosz, Thøgersen, Ida B., Enghild, Jan J., Potempa, Jan
Format: Article
Language:English
Subjects:
Annexin A1 - metabolism
Apoptosis
CD11b Antigen - metabolism
Cysteine Endopeptidases - metabolism
Cysteine Endopeptidases - physiology
Humans
Immunity, Innate
Monocytes - physiology
Neutrophils - physiology
Research Article
Staphylococcal Infections - immunology
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus - enzymology
Staphylococcus aureus - growth & development
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Summary:Circulating neutrophils and monocytes form the first line of cellular defense against invading bacteria. Here, we describe a novel and specific mechanism of disabling and eliminating phagocytes by Staphylococcus aureus. Staphopain B (SspB) selectively cleaved CD11b on phagocytes, which rapidly acquired features of cell death. SspB-treated phagocytes expressed phosphatidylserine as well as annexin I and became permeable to propidium iodide, thus demonstrating distinctive features of both apoptosis and necrosis, respectively. The cell death observed was caspase and Syk tyrosine kinase independent, whilst cytochalasin D efficiently inhibited the staphopain-induced neutrophil killing. Neutrophil and monocyte cell death was not affected by integrin clustering ligands (ICAM-1 or fibrin) and was prevented, and even reversed, by IgG. This protective effect was dependent on the Fc fragment, collectively suggesting cooperation of the CD16 receptor and integrin Mac-1 (CD11b/CD18). We conclude that SspB, particularly in the presence of staphylococcal protein A, may reduce the number of functional phagocytes at infection sites, thus facilitating colonization and dissemination of S. aureus.
ISSN:1662-811X
1662-8128
DOI:10.1159/000181014