Semisynthesis of Erythropoietin Analog Having Three Oligosaccharides

An erythropoietin (EPO) analog having three complex-type biantennary sialyloligosaccharides was prepared. The full-length EPO glycopeptide was prepared through native chemical ligation of a triglycosylated peptide- α thioester corresponding to EPO 1-32 amino acid residues, which was synthesized chem...

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Bibliographic Details
Published in:Journal of carbohydrate chemistry 2011-05, Vol.30 (4-6), p.306-319
Main Authors: Hirano, Kiriko, Izumi, Masayuki, Macmillan, Derek, Tezuka, Katsunari, Tsuji, Takashi, Kajihara, Yasuhiro
Format: Article
Language:English
Subjects:
Amino acids
Erythropoietin
Escherichia coli
Glycopeptide
Native chemical ligation
Proteins
Sialic acid
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Summary:An erythropoietin (EPO) analog having three complex-type biantennary sialyloligosaccharides was prepared. The full-length EPO glycopeptide was prepared through native chemical ligation of a triglycosylated peptide- α thioester corresponding to EPO 1-32 amino acid residues, which was synthesized chemically, and a polypeptide corresponding to EPO 33-166 amino acid residues, which was prepared by E. coli expression. Oxidative folding of the full-length glycopolypeptide afforded the biologically active EPO analog.
ISSN:0732-8303
1532-2327
DOI:10.1080/07328303.2011.604570