Use of DNA markers for cereal line uniformity assessment

Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety to meet the requirements of DUS testing is a lengthy process, particularly regarding aspects of uniformity and stability. Field testing of a...

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Bibliographic Details
Published in:Proceedings of the Latvian Academy of Sciences. Section B, Natural Sciences Natural Sciences, 2012-01, Vol.66 (1-2), p.21-25
Main Authors: Ruņgis, Dainis, Gaile, Ilze, Veinberga, Ilze, Zute, Sanita, Strazdiņa, Vija, Bleidere, Māra, Kronberga, Arta
Format: Article
Language:English
Subjects:
AFLP
Deoxyribonucleic acid
DNA
DNA markers
DUS testing
Environmental conditions
Genetic diversity
genetic uniformity
iPBS
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Summary:Prior to the registration of a new variety, it is required to undergo Distinctness, Uniformity and Stability (DUS) testing. Preparing a newly developed variety to meet the requirements of DUS testing is a lengthy process, particularly regarding aspects of uniformity and stability. Field testing of a large number of lines is time and resource intensive. In addition, the expression of certain traits may be influenced by environmental conditions. The use of DNA markers may allow rapid assessment of the level of genetic diversity within a particular line or variety, and to remove individuals that are genetically differentiated, thus accelerating the homogenisation of a newly developed variety. In this study, we utilised AFLP and the iPBS marker techniques to assess genetic variation within advanced breeding lines of several cereal species (triticale, wheat, barley). The combined use of molecular and morphological selection over three years of analysis and selection resulted in the reduction of genetic diversity within breeding lines. Lai registrētu jaunu šķirni, tai ir nepieciešams iziet atšķirīguma, viendabīguma un stabilitātes (AVS) testu. Jaunas šķirnes izveidošana atbilstoši AVS testa prasībām ir ilgstošs process, īpaši viendabīguma un stabilitātes aspektā. Lauka pārbaudes lielam skaitam līniju ir laika un resursu ietilpīgas, un vides apstākļi var ietekmēt fenotipiskās īpašības. Izmantojot DNS marķierus, ir iespējams noteikt genētisko daudzveidību selekcijas līnijās un atlasīt indivīdus, kuri uzrāda paaugstinātu genētisko diferenciāciju, tā paātrinot jaunās šķirnes genētisko izlīdzināšanu. Šajā pētījumā tika izmantoti AFLP un iPBS marķieri, lai noteiktu genētisko daudzveidību dažādu graudaugu (tritikāle, kvieši, mieži, auzas) selekcijas līnijās. Izmantojot molekulāro un morfologisko izlasi, trīs gadu laikā tika panākta genētiskās daudzveidības samazināšanās analizētajās selekcijas līnijās.
ISSN:1407-009X
2255-890X
DOI:10.2478/v10046-011-0041-1