Ribosomal protein S1 functions as a termination factor in RNA synthesis by Q[beta] phage replicase

S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved i...

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Bibliographic Details
Published in:Nature communications 2013-04, Vol.4, p.1781
Main Authors: Vasilyev, Nikita N, Kutlubaeva, Zarina S, Ugarov, Victor I, Chetverina, Helena V, Chetverin, Alexander B
Format: Article
Language:English
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Summary:S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved in later events. Here, we show that in Qβ replicase-mediated RNA synthesis, S1 functions at the termination step by promoting release of the product strand in a single-stranded form. This function is fulfilled by the N-terminal fragment comprising the first two S1 domains. The results suggest that S1 might also have a role other than mRNA binding in the ribosome.
ISSN:2041-1723
DOI:10.1038/ncomms2807