Characterization of precipitates formed by H2S-producing, Cu-resistant Firmicute isolates of Tissierella from human gut and Desulfosporosinus from mine waste

The purpose of this study was to characterize precipitates formed in anaerobic, H 2 S-producing cultures of two Tissierella isolates and Desulfosporosinus strain DB. The cultures were grown in Cu-containing media as part of a larger study of Cu resistance in anaerobic sulfidogens. The Tissierella st...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2013-06, Vol.103 (6), p.1221-1234
Main Authors: Ikkert, Olga P., Gerasimchuk, Anna L., Bukhtiyarova, Polina A., Tuovinen, Olli H., Karnachuk, Olga V.
Format: Article
Language:English
Subjects:
Biogeochemistry
Biomedical and Life Sciences
Chemical composition
Copper
Hydrogen sulfide
Iron compounds
Iron phosphates
Life Sciences
Medical Microbiology
Microbiology
Mine wastes
Original Paper
Peptones
Phosphates
Plant Sciences
Soil Science & Conservation
Sulfate reduction
Sulfide compounds
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Summary:The purpose of this study was to characterize precipitates formed in anaerobic, H 2 S-producing cultures of two Tissierella isolates and Desulfosporosinus strain DB. The cultures were grown in Cu-containing media as part of a larger study of Cu resistance in anaerobic sulfidogens. The Tissierella strains produced H 2 S from peptone. Desulfosporosinus formed H 2 S from peptone or through dissimilatory sulfate reduction with lactate. Tissierella cultures precipitated iron phosphate, vivianite, but no crystalline phases or Cu sulfides were detected. Multiple Cu sulfides, including chalcopyrite and covellite, were detected in Desulfosporosinus cultures but vivianite was not formed. Ion microprobe spectra and electron microscopic examination showed major variation in the elemental composition and morphological differences depending on incubation conditions. Extended incubation time for at least 1–2 months increased the crystallinity of the precipitates. The results highlight biogeochemical differences in sulfide and phosphate precipitates between the two major groups of Firmicutes although they may share the same habitat including the human intestinal tract.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-013-9900-x