Oxidative DNA damage and [beta]-catenin expression in colorectal cancer evolution

Purpose Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with [beta]-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and [beta]-catenin ex...

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Bibliographic Details
Published in:International journal of colorectal disease 2013-05, Vol.28 (5), p.713
Main Authors: Priolli, Denise G, Canelloi, Thamy P, Lopes, Camila O, Valdívia, Júlio C. M, Ma, Açari, Demetrius P, Cardinalli, Izilda A
Format: Article
Language:English
Subjects:
Analysis
Cancer
Colorectal cancer
DNA
DNA damage
Gastrointestinal diseases
Genetic aspects
Genetic research
Immunohistochemistry
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Summary:Purpose Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with [beta]-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and [beta]-catenin expression in normal mucosa and colon tumor tissue (adenoma and adenocarcinoma) in colorectal adenocarcinoma evolution. Method One hundred and 13 samples were studied. Hematoxylin-eosin determined histological grade. [beta]-Catenin expression was analyzed by immunohistochemistry. The oxidative DNA damage was evaluated using comet assay technique. The coefficient for rejection of the nullity hypothesis was taken to 5 %. Kruskal-Wallis, Spearman test, and partial correlation were used to analyze the data. Results There was oxidative DNA damage increase in colorectal cancer evolution (p < 0.01). Histological grade was correlated with oxidative DNA damage (p < 0.01). There were differences in [beta]-catenin expression among normal, adenoma, and adenocarcinoma tissue with progressive increase of [beta]-catenin expression (p < 0.00). Histological grade was correlated to [beta]-catenin expression (p < 0.00). There was a relationship (p < 0.00) between [beta]-catenin and histological grade while controlling for the effect of oxidative DNA damage. Conclusion The findings of this study make it possible to establish a relationship between oxidative DNA damage and [beta]-catenin expression in normal mucosa and colorectal tumor tissue. Additionally, they show a causal relationship between variations of [beta]-catenin in different tissues analyzed while controlling for the effect of oxidative DNA damage.
ISSN:0179-1958
1432-1262
DOI:10.1007/s00384-013-1688-7