A Fluorogenic Probe for [gamma]-Glutamyl Cyclotransferase: Application of an Enzyme-Triggered O-to-N Acyl Migration-Type Reaction

Light it up: human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here...

Full description

Saved in:
Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2013-11, Vol.14 (16), p.2110
Main Authors: Yoshiya, Taku, Tsuda, Shugo, Mochizuki, Masayoshi, Hidaka, Koushi, Tsuda, Yuko, Kiso, Yoshiaki, Kageyama, Susumu, Ii, Hiromi, Yoshiki, Tatsuhiro, Nishiuchi, Yuji
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Light it up: human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA-4, which should contribute toward further understanding of GGCT.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201300481