The association between heat shock protein P2/P2 genotype with Egyptian hepatocellular carcinoma patients

• Published in: Comparative clinical pathology 2013-11, Vol.22 (6), p.1183-1188
• Main Authors: ElGemei, Shahira S., Shaheen, Noha M., Abu Khalil, Reham Emad
• Format: Article
• Language: English
• Subjects: Hematology; Medicine; Medicine & Public Health; Oncology; Original Article; Pathology
• ISSN: 1618-5641; 1618-565X
• DOI: 10.1007/s00580-012-1547-x

Polymorphism of heat shock protein 70 (HSP70) at position 1267 may influence its expression. Many studies showed the importance of this polymorphism in developing cancers and some autoimmune diseases. In this study, we investigated the association of this polymorphism with the risk of hepatocellular carcinoma (HCC) in Egyptian patients. The study included 40 cases with HCC and 20 healthy age- and sex-matched subjects as control. The cases included 31 males (77.5 %) and 9 females (22.5 %). Their ages ranged from 42 to 70 years. HSPA1B 1267 was investigated using polymerase chain reaction with restriction fragment length polymorphism cutting with PstI enzyme. The two allelic forms were designated as the P1 allele and P2 allele. Among the HCC group, 14 patients (35 %) had P2/P2 genotype, 15 patients (37.5 %) had P1/P2 genotype, and 11 patients (27.5 %) had P1/P1 genotype. The frequencies of the HSPA1B P2/P2 genotype and the HSPA1B P2 allele in HCC patients were higher than in controls (each p  = 0.0001). The risk for developing HCC in the homozygous HSPA1B P2/P2 genotype was 2.8-fold higher than that in the heterozygous P1/P2 genotype. In conclusion, polymorphism of HSP70 at position 1267 may be a risk for HCC development in Egyptians.