Studies on Pharmacological Activation of Human Serum Immunoglobulin G by Chemical Modification and Active Subfragments. IX. Anti-inflammatory Activity of Completely Alkylated Heavy Chain (C.Fr.I-H)

• Published in: Biological & pharmaceutical bulletin 1993/03/15, Vol.16(3), pp.309-311
• Main Authors: MIMURA, Tsutomu, ITOH, Masaaki, HARUYAMA, Munetada, SUGIYAMA, Tetsuya, SUZUKI, Nobutaka, TSUJIKAWA, Kazutake, KOHAMA, Yasuhiro
• Format: Article
• Language: English
• Subjects: Alkylation; Amino Acids - analysis; Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; carboxamide-methylated heavy chain; Carrageenan; carrageenin paw edema; carrageenin pleurisy; complete alkylation; Disulfides - chemistry; Edema - chemically induced; Edema - prevention & control; Electrophoresis, Polyacrylamide Gel; human IgG; Immunoglobulin G - chemistry; Immunoglobulin G - immunology; Male; Medical sciences; Mice; Mice, Inbred Strains; Peptide Fragments - pharmacology; Pharmacology. Drug treatments; Pleurisy - chemically induced; Pleurisy - prevention & control; Rats; Rats, Sprague-Dawley; Rats, Wistar; Serotonin; serotonin paw edema
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.16.309

The anti-inflammatory activity of completely reduced and carboxamide-methylated heavy chain of all disulfide bonds in human serum immunoglobulin G (IgG) (C.Fr.I-H) was investigated using some experimental inflammatory models. C.Fr.I-H significantly inhibited carrageenin-induced paw edema and pleurisy, and serotonin-induced paw edema. The carboxamide-methylated heavy chain of interchain disulfide bonds in IgG (Fr.I-H) exhibited similar inhibitory activities to C.Fr.I-H on carrageenin-induced paw edema and pleurisy, while it was ineffective on serotonin-induced paw edema. These results suggest that complete alkylation of disulfide bonds in a heavy chain caused stronger anti-inflammatory activities than partial alkylation.