Involvement of TNF-[alpha] in differential gene expression pattern of CXCR4 on human marrow-derived mesenchymal stem cells

• Published in: Molecular biology reports 2014-02, Vol.41 (2), p.1059
• Main Authors: Ziaei, Rozita, Ayatollahi, Maryam, Yaghobi, Ramin, Sahraeian, Zeinab, Zarghami, Nosratollah
• Format: Article
• Language: English
• Subjects: Chemokines; Gene expression; Neurons; Stem cells; Tumors
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-013-2951-2

Cell therapy and tissue repair are used in a variety of diseases including tissue and organ transplantation, autoimmune diseases and cancers. Now mesenchymal stem cells (MSCs) are an attractive and promising source for cell-based therapy according to their individual characteristics. Soluble factors which are able to induce MSCs migration have a vital role in cell engraftment and tissue regeneration. Tumor necrosis factor [alpha] (TNF-[alpha]) is a major cytokine present in damaged tissues. We have investigated the pattern of gene expression of chemokine receptor CXCR4 in nine groups of human bone marrow-derived MSCs stimulated with TNF-[alpha] in different dose and time manner. Comparison of TNF-[alpha] treated with untreated MSCs revealed the highest expression level of CXCR4 after treatment with 1, and 10 ng/ml of TNF-[alpha] in 24 h, and the production of CXCR4 mRNA was regulated up to 216 and 512 fold, respectively. Our results demonstrated the differential gene expression pattern of chemokine receptor CXCR4 in human marrow-derived MSCs stimulated with inflammatory cytokine TNF-[alpha]. These findings suggest that in vitro control of both dose and time factors may be important in stem cell migration capacity, and perhaps in future-stem cell transplantation therapies.[PUBLICATION ABSTRACT]