Aberrant activation of Sonic hedgehog signaling in chronic cholecystitis and gallbladder carcinoma

• Published in: Human pathology 2014-03, Vol.45 (3), p.513-521
• Main Authors: Xie, Fang, PhD, Xu, Xiaoping, PhD, Xu, Angao, PhD, Liu, Cuiping, PhD, Liang, Fenfen, MS, Xue, Minmin, MS, Bai, Lan, PhD
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Carcinoma - metabolism; Carcinoma - mortality; Carcinoma - pathology; Cholecystitis - metabolism; Cholecystitis - mortality; Cholecystitis - pathology; Chronic cholecystitis; Female; Gallbladder - metabolism; Gallbladder - pathology; Gallbladder carcinoma; Gallbladder diseases; Gallbladder Neoplasms - metabolism; Gallbladder Neoplasms - mortality; Gallbladder Neoplasms - pathology; Gli1; Hedgehog Proteins - metabolism; Hospitals; Humans; Liver cirrhosis; Lymphocytes; Male; Medical prognosis; Middle Aged; Mucous Membrane - metabolism; Mucous Membrane - pathology; Mutation; Pancreatic cancer; Pathology; Proteins; Ptch; Rodents; Signal Transduction - physiology; Sonic hedgehog pathway; Studies; Survival Rate
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2013.10.017

Summary Sonic hedgehog (Shh) signaling has been extensively studied and is implicated in various inflammatory diseases and malignant tumors. We summarized the clinicopathological features and performed immunohistochemistry assays to examine expression of Shh signaling proteins in 10 normal mucosa, 32 gallbladder carcinoma (GBC), and 95 chronic cholecystitis (CC) specimens. The CC specimens were classified into three groups according to degree of inflammation. Compared with normal mucosa, CC, and GBC specimens exhibited increased expression of Shh. The immunoreactive score of Shh in the GBC group was higher than that in the mild to moderate CC groups but lower than that in the severe CC group ( P < .05). Expression of Patched (Ptch) and Gli1 gradually increased from non-malignant cholecystitis to malignant tumors. Compared with CC specimens, GBC specimens showed higher cytoplasmic and membranous expression for Ptch ( P < .05). Gli1 staining showed cytoplasmic expression of Gli1 in both CC (60% for mild, 77% for moderate, and 84% for severe) and GBC specimens (97%). Nuclear expression of Gli1 was detected in 16% of severe CC specimens with moderate to poor atypical hyperplasia, and in 62.5% of GBC specimens. Shh expression strongly correlated with expression of Ptch and Gli1. Furthermore, patients with strongly positive Gli1 staining had significantly lower survival rates than those with weakly positive staining. Our data indicate that the Shh signaling pathway is aberrantly activated in CC and GBC, and altered Shh signaling may be involved in the course of development from CC to gallbladder carcinogenesis.