Gamma radiation-induced human B cell defects: Model for space flight

• Published in: Journal of allergy and clinical immunology 2004-02, Vol.113 (2), p.S42-S42
• Main Authors: Nance, C.L., Shearer, W.T.
• Format: Article
• Language: English
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2003.12.112

One of the most feared space flight conditions is radiation due to the potential to weaken the immune system. Leukocyte studies in space demonstrated a reduction in proliferative responses, alterations in signal transduction and cytokine production, along with reactivation of latent viruses. Utilizing our previously developed human B cell in vitro model, we present evidence of space-like gamma radiation-induced B cell defects. CD40-activated purified human peripheral blood B cells were exposed to varying levels of gamma radiation (1-6 Gy) delivered by a 137Cs source. Determination of B cell responses and apoptotic processes were made by flow cytometric analysis, ELISA, colorometric, protein, and proliferative assays. Human B cells were significantly altered by 3 Gy gamma radiation (estimated radiation absorbed by humans on a two-year space journey). There was significant reduction in cell viability (40%), proliferation (70%), and CD79b (B cell receptor) expression (50%) ( p