Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl- N′-nitro- N-nitrosoguanidine in Caco-2 and V79 cell lines

Since N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of N-nitrosomorpholine (NMOR) and N-methyl- N′-nitro- N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Ca...

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Bibliographic Details
Published in:Cancer letters 2002-08, Vol.182 (1), p.11-18
Main Authors: ROBICHOVA, Sona, SLAMENOVA, Darina
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Ascorbic Acid - pharmacology
Biological and medical sciences
Caco-2 Cells
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens - toxicity
Cricetinae
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA - biosynthesis
DNA Damage
DNA methylation
Dose-Response Relationship, Drug
Ethanol
Foods and miscellaneous
Genotoxicity
Humans
Medical research
Medical sciences
Membrane filters
Methylnitronitrosoguanidine - toxicity
Mitochondrial DNA
N-methyl- N′-nitro- N-nitrosoguanidine
N-nitrosomorpholine
Nitrosamines - toxicity
Tumors
Vitamin C
Vitamin E
Vitamin E - pharmacology
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Summary:Since N-nitroso compounds as strong carcinogens are closely related to food and nutrition, the cytotoxic effects of N-nitrosomorpholine (NMOR) and N-methyl- N′-nitro- N-nitrosoguanidine (MNNG) and their reduction by vitamins C and E were investigated in hamster V79 cells and human colon carcinoma Caco-2 cells. Cytotoxicity was evaluated by the trypan blue exclusion technique in Caco-2 cells and by the plating efficiency assay in V79 cells. NMOR caused a dose-dependent decline of viable cells in both cell lines; MNNG induced a dose-dependent cytotoxic effect only in V79 cells. Pretreatment of cells with vitamin C and vitamin E significantly reduced the cytotoxicity of NMOR, however, both vitamins had not effect on cytotoxicity induced by MNNG. These results suggest that different N-nitroso compounds react differently with cellular macromolecules. Measurement of the level of NMOR-induced DNA strand breaks and alkali-labile sites in both cell types using the alkaline comet assay also indicates a protective effect of both vitamins against the genotoxic effects of NMOR.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(02)00056-3