Regulated expression of leukocyte-specific transcript (LST) 1 in human intestinal inflammation

• Published in: Inflammation research 2014-07, Vol.63 (7), p.513-517
• Main Authors: Heidemann, Jan, Kebschull, Moritz, Tepasse, Phil Robin, Bettenworth, Dominik
• Format: Article
• Language: English
• Subjects: Adult; Allergology; Anti-Inflammatory Agents, Non-Steroidal - adverse effects; Biomedical and Life Sciences; Biomedicine; Caco-2 Cells; Cells, Cultured; Colitis - etiology; Colitis - genetics; Colitis - metabolism; Colitis - pathology; Colon - metabolism; Colon - pathology; Dermatology; Diverticulitis - complications; Diverticulitis - genetics; Diverticulitis - metabolism; Diverticulitis - pathology; Endothelial Cells - metabolism; Female; Gene Expression; Humans; Immunology; Inflammatory Bowel Diseases - genetics; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; Intestinal Mucosa - metabolism; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Middle Aged; Neurology; Pharmacology/Toxicology; Rheumatology; Short Communication; Young Adult
• ISSN: 1023-3830; 1420-908X
• DOI: 10.1007/s00011-014-0732-6

Introduction Leukocyte-specific transcript 1 (LST1) encoded peptides are involved in immunomodulation and nanotube-mediated cell–cell communication. The aim of this study was to assess the expression of LST1 in colonic epithelium and endothelium during intestinal inflammation. Methods LST1 expression was evaluated by RT-PCR, FACS, western blot analysis, and immunohistochemistry in intestinal epithelial Caco-2 cells, human intestinal microvascular endothelial cells and in human histological specimens from inflammatory bowel disease (IBD) patients and non-IBD colitis patients. Results LST1 expression was significantly increased upon proinflammatory stimulation in intestinal epithelial and endothelial cells. Furthermore, LST1 tissue expression was significantly enhanced in macroscopically inflamed colonic mucosal biopsies as compared to non-affected mucosal areas. Conclusions This is the first report demonstrating regulated LST1 expression in human intestinal epithelial and microvascular endothelial cells and in inflamed colonic tissue from IBD patients. Proinflammatory expression of LST1 occurs in the setting of human IBD and is not restricted to immune cell populations. Future studies are needed to further elucidate the role of soluble and membrane-expressed LST1 in the regulation of mucosal intestinal immunity and inflammation as well as to reveal possible therapeutic implications.