Silicon nutrition alleviates the negative impacts of arsenic on the photosynthetic apparatus of rice leaves: an analysis of the key limitations of photosynthesis

• Published in: Physiologia plantarum 2014-10, Vol.152 (2), p.355-366
• Main Authors: Sanglard, Lílian M. V. P, Martins, Samuel C. V, Detmann, Kelly C, Silva, Paulo E. M, Lavinsky, Alyne O, Silva, Mariela M, Detmann, Edenio, Araújo, Wagner L, DaMatta, Fábio M
• Format: Article
• Language: English
• Subjects: abiotic stress; Analysis of Variance; Arsenic; Arsenic - toxicity; arsenites; carbon; Carbon Dioxide - metabolism; chlorophyll; cultivars; Diffusion; fluorescence; gas exchange; Genotype; leaf conductance; leaves; mesophyll; mutants; Nutrition; Oryza - drug effects; Oryza - genetics; Oryza - physiology; Oryza sativa; Photosynthesis; Photosynthesis - drug effects; Plant Leaves - drug effects; Plant Leaves - physiology; Plant Stomata - drug effects; Plant Stomata - physiology; rice; roots; silicon; Silicon - pharmacology; stomatal conductance; Time Factors; toxicity
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.12178

Silicon (Si) plays important roles in alleviating various abiotic stresses. In rice (Oryza sativa), arsenic (As) is believed to share the Si transport pathway for entry into roots, and Si has been demonstrated to decrease As concentrations. However, the physiological mechanisms through which Si might alleviate As toxicity in plants remain poorly elucidated. We combined detailed gas exchange measurements with chlorophyll fluorescence analysis to examine the effects of Si nutrition on photosynthetic performance in rice plants [a wild‐type (WT) cultivar and its lsi1 mutant defective in Si uptake] challenged with As (arsenite). As treatment impaired carbon fixation (particularly in the WT genotype) that was unrelated to photochemical or biochemical limitations but, rather, was largely associated with decreased leaf conductance at the stomata and mesophyll levels. Indeed, regardless of the genotypes, in the plants challenged with As, photosynthetic rates correlated strongly with both stomatal (r² = 0.90) and mesophyll (r² = 0.95) conductances, and these conductances were, in turn, linearly correlated with each other. The As‐related impairments to carbon fixation could be considerably reverted by Si in a time‐ and genotype‐dependent manner. In conclusion, we identified Si nutrition as an important target in an attempt to not only decrease As concentrations but also to ameliorate the photosynthetic performance of rice plants challenged with As.