Not all sugars are sweet for banana multiplication. In vitro multiplication, rooting, and acclimatization of banana as influenced by carbon source-concentration interactions

Carbon sources have been considered as one of the most important factors for in vitro multiplication of excised tissues. The type and concentration of sugars are known to influence the success of any in vitro protocol. In the present investigation, the effect of four carbon sources (glucose, fructos...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2014-10, Vol.50 (5), p.552-560
Main Authors: Waman, Ajit Arun, Bohra, Pooja, Sathyanarayana, B. N
Format: Article
Language:English
Subjects:
acclimation
Acclimatization
bananas
Biomedical and Life Sciences
Carbon
Carbon sources
Cell Biology
Chlorophyll
Developmental Biology
fructose
Fruits
genome
Glucose
Indian culture
Leaves
Life Sciences
mannitol
Micropropagation
Multiplication & division
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant roots
Plant Sciences
Plantlets
Plants
Rooting
shoots
Sucrose
Sugar
Sugars
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Carbon sources have been considered as one of the most important factors for in vitro multiplication of excised tissues. The type and concentration of sugars are known to influence the success of any in vitro protocol. In the present investigation, the effect of four carbon sources (glucose, fructose, mannitol, and sucrose) used to supplement Murashige and Skoog medium at three concentrations (1, 2, and 3%) was studied with respect to in vitro multiplication, in vitro rooting, and hardening in ‘Silk’ banana. Fructose (2%) followed by sucrose (3%) were found to be most the congenial carbon sources for obtaining the highest shoot multiplication rates. Growth parameters were also found to be superior in the fructose-containing media; however, it performed poorly in terms of root induction. Whereas, medium containing sucrose (3%) supported 100% root induction of in vitro-derived shoots. Plantlets multiplied initially on glucose-containing (1 and 3%) and sucrose-containing (3%) media exhibited superior growth parameters after secondary hardening. We conclude that a change in the rooting media could improve the number of successfully hardened plants under ex vitro conditions. To our knowledge, this is the first report describing the effect of sugars on hardening-related parameters in AAB genome of banana and apparently on any variety of banana.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-014-9623-3