Development and validation of stability indicating RP-HPLC method for simultaneous estimation of Atenolol and Nifedipine in bulk and pharmaceutical dosage form

• Published in: Asian Journal of Research in Chemistry 2015-02, Vol.8 (2), p.91
• Main Authors: Saravanan, Govindaraj, Katari, Sannihith, Krishnanjaneyulu, Immadisetty Sri, Visagaperumal, Dhanapal
• Format: Article
• Language: English
• ISSN: 0974-4169; 0974-4150
• DOI: 10.5958/0974-4150.2015.00017.6

A simple, rapid, selective, sensitive, linear, precise and accurate stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Atenolol and Nifedipine in pharmaceutical dosage form. Separation was attained on a STD Kromasil C18 column at 30 degrees Celsius, using a mobile phase consisting of phosphate buffer (pH 5.0) and acetonitrile in the ratio of 45: 55 % v/v, at a flow rate of 1.0 ml/min. The UV detection wavelength was 232 nm and 10 μl of sample was injected. The linearity was found to be 15-90 μg/ml for Atenolol and 6-36 μg/ml for Nifedipine with a correlation coefficient of 0.9993 and 0.9994, respectively. Retention times were found to be 2.474 min and 4.553 min for Atenolol and Nifedipine, respectively. The overall mean % recoveries were found to be 99.69% for Atenolol and 99.68% for Nifedipine. The % RSD for precision, robustness and ruggedness of the proposed method was found to be less than 2%.