Diversity of Clinical Implication of B-Cell Translocation Gene 1 Expression by Histopathologic and Anatomic Subtypes of Gastric Cancer
Background Genetic signatures may differ by histopathologic and anatomic subtypes of gastric cancer (GC). B-cell translocation gene 1 ( BTG1 ) was identified as one of genes downregulated in GC tissues from our microarray data. Aims To evaluate the clinical implications of BTG1 expression in GC and...
Saved in:
Published in: | Digestive diseases and sciences 2015-05, Vol.60 (5), p.1256-1264 |
---|---|
Main Authors: | , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Background
Genetic signatures may differ by histopathologic and anatomic subtypes of gastric cancer (GC). B-cell translocation gene 1 (
BTG1
) was identified as one of genes downregulated in GC tissues from our microarray data.
Aims
To evaluate the clinical implications of
BTG1
expression in GC and the genetic diversity among GC subtypes.
Methods
BTG1
mRNA expression was analyzed in GC cell lines and 233 pairs of surgical specimens. The mutational and methylation status of
BTG1
in GC cell lines was analyzed, and immunohistochemistry was conducted to determine the distribution of BTG1. The pattern and prognostic significance of BTG1 expression were correlated with the three proposed GC subtypes.
Results
BTG1
mRNA was downregulated in 82 % of GC cell lines and in 88 % of clinical GC tissues. Promoter hypermethylation events or sequence mutations were not detected in GC cell lines. The pattern of BTG1 expression as observed by immunohistochemistry was consistent with that of its mRNA. Downregulation of
BTG1
mRNA in GCs was significantly associated with shorter disease-specific and recurrence-free survival. Multivariate analysis of disease-specific survival identified downregulation of
BTG1
transcription as an independent prognostic factor.
BTG1
mRNA expression was more strongly suppressed in proximal nondiffuse and diffuse GC compared with distal nondiffuse GC, and subgroup analysis revealed that
BTG1
downregulation led to adverse prognosis, specifically in patients with proximal nondiffuse and diffuse GC.
Conclusions
Altered expression of
BTG1
is a potential biomarker for carcinogenesis and progression of GC, particularly for proximal nondiffuse and diffuse GC. |
---|---|
ISSN: | 0163-2116 1573-2568 |
DOI: | 10.1007/s10620-014-3477-8 |