AFM-based protein fishing in the pulsed electric field

A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase t...

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Bibliographic Details
Published in:Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2015-04, Vol.9 (2), p.121-129
Main Authors: Ivanov, Yu. D., Pleshakova, T. O., Malsagova, K. A., Kaysheva, A. L., Kopylov, A. T., Izotov, A. A., Tatur, V. Yu, Vesnin, S. G., Ivanova, N. D., Ziborov, V. S., Archakov, A. I.
Format: Article
Language:English
Subjects:
Bioorganic Chemistry
Biophysics
Chemistry
Chemistry and Materials Science
Electric fields
Mass spectrometry
Medicinal Chemistry
Microscopy
Proteins
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Summary:A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase the AFM-fishing efficiency we have applied pulsed voltage with the rise time of the front of about 1 ns to the AFM chip. The AFM-chip was made using a conductive material, highly oriented pyrolytic graphite (HOPG). The increased efficiency of AFM-fishing has been demonstrated using detection of cytochrome b 5 protein. Selection of the stimulating pulse with a rise time of 1 ns, corresponding to the GHz frequency range, by the effect of intrinsic emission from water observed in this frequency range during water injection into the cell.
ISSN:1990-7508
1990-7516
DOI:10.1134/S1990750815020080