Directed Evolution and Structural Analysis of Alkaline Pectate Lyase from the Alkaliphilic Bacterium Bacillus sp. Strain N16-5 To Improve Its Thermostability for Efficient Ramie Degumming

Thermostable alkaline pectate lyases have potential applications in the textile industry as an alternative to chemical-based ramie degumming processes. In particular, the alkaline pectate lyase from Bacillus sp. strain N16-5 (BspPelA) has potential for enzymatic ramie degumming because of its high s...

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Bibliographic Details
Published in:Applied and environmental microbiology 2015-09, Vol.81 (17), p.5714-5723
Main Authors: Zhou, Cheng, Ye, Jintong, Xue, Yanfen, Ma, Yanhe
Format: Article
Language:English
Subjects:
Alkalies - metabolism
Amino Acid Sequence
Bacillus - chemistry
Bacillus - enzymology
Bacillus - genetics
Bacteria
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Crystallography, X-Ray
Directed Molecular Evolution
Enzyme Stability
Enzymology and Protein Engineering
Hot Temperature
Kinetics
Models, Molecular
Molecular Sequence Data
Mutagenesis
Mutants
Mutation
Polymerase chain reaction
Polysaccharide-Lyases - chemistry
Polysaccharide-Lyases - genetics
Polysaccharide-Lyases - metabolism
Simulation
Simulation analysis
Structural analysis
Textile industry
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