Effect of various techniques of tetracycline hydrochloride demineralization on root dentin surface: A scanning electron microscopic study

• Published in: Scholars research journal 2011-07, Vol.1 (2)
• Main Authors: Bhardwaj, Amit, Madhumala, R, Thiruneervannan, R, Verma, Shalu
• Format: Article
• Language: English
• ISSN: 2249-5975; 0976-8459
• DOI: 10.4103/2249-5975.99656

Aim : To evaluate the in-vitro effect of various techniques of Tetracycline Hydrochloride application on root dentin surface at different magnifications of scanning electron microscopic (SEM). Materials and Methods : Twenty human teeth were collected and stored in distilled water after soft tissue removal using hand instruments. The apical third of each root was removed remaining mid-third region was sectioned longitudinally through the root canal following removal of its cementum with a tapered fissure bur. The dentin blocks were divided into 4 groups having 5 samples in each group. A tetracycline hydrochloride solution was prepared by adding the contents of one 500 mg capsule to 5 ml of distilled water. The application time of the tetracycline hydrochloride was 5 minutes. After the treatment of the root surfaces, the samples were fixed in 2.5% glutaraldehyde prepared in phosphate buffer (pH 7.3) for 24 hours at 4C and prepared for SEM. Mean and standard deviation were estimated from the samples for each study group. Mean values were compared by either ONE-WAY ANOVA with Tukey - HSD procedure. Results : All technique of tetracycline application shows removal of smear layer and exposure of dentinal tubule orifices. Burnishing technique showed significant difference than immersion technique and saturation technique. Conclusion : In view of these present findings, tetracycline hydrochloride as a root conditioner may be evaluated in vivo studies. It is reasonable to expect lesser tissue destruction and better regeneration of periodontal tissues if it is used as a root conditioner during periodontal regeneration procedures.