Morphological and molecular characterization of the second backcross progenies of Ogu-CMS Chinese kale and rapeseed

• Published in: Euphytica 2017-02, Vol.213 (2), p.1, Article 55
• Main Authors: Yu, Hai-long, Li, Zhi-yuan, Yang, Li-mei, Liu, Yu-mei, Zhuang, Mu, Zhang, Lu-gang, Lv, Hong-hao, Li, Zhan-sheng, Han, Feng-qing, Liu, Xiao-ping, Fang, Zhi-yuan, Zhang, Yang-yong
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biotechnology; Fertility; Hybrids; Life Sciences; Male sterility; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Pollen; Pollination
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-017-1842-3

Ogura cytoplasmic male sterility (Ogu-CMS) is widely used in the production of commercial hybrids of Brassica oleracea . However, the widespread application of the Ogu-CMS system in B. oleracea has hindered the germplasm innovation of Ogu-CMS resources due to the lack of a natural restorer line. Previously, the Ogu-CMS fertility-restored interspecific hybrids between rapeseed 15Y403 (2n = 38, AACC) and Chinese kale JL1 (2n = 18, CC) have been successfully produced. However, these progenies, which still contained a large proportion of rapeseed genomic components, showed poor fertility and a low seed setting rate under natural pollination. To improve fertility and seed setting, a successive backcross with JL1 was performed to produce BC 2 progenies. Screening with the Rfo -specific marker, five individuals harboring the Rfo gene were identified among 98 BC 2 progenies. These five individuals underwent background marker screening and an evaluation of agronomic traits and fertility. One individual (code: 15Q23) was identified with higher pollen viability, better seed setting under natural pollination, and a closer genetic background to the parent Chinese kale JL1. Many morphological traits showed no significant differences ( P