Pharmacokinetic profiles contribute to the differences in behavioral pharmacology of 071031B enantiomers as novel serotonin and norepinephrine reuptake inhibitors

• Published in: Journal of psychopharmacology (Oxford) 2017-03, Vol.31 (3), p.377-386
• Main Authors: Xue, Rui, Li, Ying, He, Xin-Hua, Jin, Zeng-Liang, Fan, Shi-Yong, Zhang, Ting-Ting, Li, Nuo-Min, Yuan, Li, Zheng, Ai-Ping, Zhong, Bo-Hua, Li, Yun-Feng, Zhang, You-Zhi
• Format: Article
• Language: English
• Subjects: Acetic acid; Affinity; Animal models; Animals; Antidepressants; Antidepressive Agents - pharmacokinetics; Antidepressive Agents - pharmacology; Behavior, Animal - drug effects; Benzodioxoles - pharmacokinetics; Benzodioxoles - pharmacology; Catecholamines; Depression; Depression - drug therapy; Depression - metabolism; Depressive Disorder - drug therapy; Depressive Disorder - metabolism; Duloxetine; Duloxetine Hydrochloride - pharmacokinetics; Duloxetine Hydrochloride - pharmacology; Enantiomers; Exploration; Formaldehyde; Intestine; Liver; Male; Mental depression; Metabolism; Mice; Mice, Inbred ICR; Noradrenaline; Norepinephrine; Norepinephrine - metabolism; Norepinephrine Plasma Membrane Transport Proteins - pharmacokinetics; Norepinephrine Plasma Membrane Transport Proteins - pharmacology; Pain; pH effects; Pharmacokinetics; Pharmacology; Rats; Rats, Sprague-Dawley; Rodents; Serotonin; Serotonin - metabolism; Serotonin and Noradrenaline Reuptake Inhibitors - pharmacokinetics; Serotonin and Noradrenaline Reuptake Inhibitors - pharmacology; Serotonin Plasma Membrane Transport Proteins - metabolism; Swimming; Swimming - physiology; Thiophenes - pharmacokinetics; Thiophenes - pharmacology
• ISSN: 0269-8811; 1461-7285
• DOI: 10.1177/0269881116681456

Our previous study indicated that a chiral compound 071031B was a novel serotonin and noradrenaline reuptake inhibitor with superior antidepressant activity compared to duloxetine. The present study aimed to investigate chiral pharmacology differences of 071031B enantiomers, S-071031B and R-071031B, and disclose mechanisms underlying the behavioral differences based on target profiles and pharmacokinetic profiles. In vivo behavioral tests indicated that S-071031B was more potent than R-071031B in two depression models (the forced swimming test in mice and rats) and two pain models (the acetic acid-induced writhing and formalin tests in mice). In vitro assays revealed that both S-071031B and R-071031B showed high affinity for human serotonin transporters and norepinephrine transporters with equal potency, and showed consistently equipotent inhibitory effects on serotonin and norepinephrine uptake. Pharmacokinetic studies demonstrated that oral availability and hepatic metabolism, rather than pH stability, intestinal transport, and plasma binding, contributed to enantiomers’ behavioral differences. Based on these findings, it is suggested that S-071031B is a more active enantiomer, and the differential pharmacokinetic profiles, but not target affinity, contribute to differences of S-071031B and R-071031B in behavioral pharmacology. Moreover, current PK-PD study may provide positive exploration for chiral antidepressants development.