Screening of bacterial genes responsible for resistance to beta-lactam antibiotics using microarrays with enzymatic detection

• Published in: Moscow University chemistry bulletin 2016-07, Vol.71 (4), p.236-242
• Main Authors: Ulyashova, M. M., Presnova, G. V., Pobolelova, Yu. I., Filippova, A. A., Egorov, A. M., Rubtsova, M. Yu
• Format: Article
• Language: English
• Subjects: Amides; Antibiotics; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Genes; Peroxidase; Subgroups
• ISSN: 0027-1314; 1935-0260
• DOI: 10.3103/S002713141604009X

The method of hybridization analysis on microarrays with enzymatic detection based on horseradish peroxidase is applied to screen infectious agents of nosocomial and community-acquired infections for beta-lactamase genes causing resistance to beta-lactam antibiotics. The advantages of using this method for the rapid identification of genes are demonstrated. Similarities and differences in the distribution of beta-lactamase genes identified in the infectious agents of nosocomial and community-acquired infections are revealed. The most common type of extended-spectrum beta-lactamases (ESBLs) is CTX-M. The high prevalence of extended-spectrum beta-lactamases, particularly of the TEM-1 beta-lactamase, is demonstrated. Individually or in combination with genes of TEM-1 and SVH-1 beta-lactamases, the genes of subgroup CTX-M-1 beta-lactamases were the most frequently identified in community-acquired infectious agents. There were no cases of the simultaneous detection of multiple ESBLs in community-acquired infectious agents. Much more varied combinations of beta-lactamases were identified in nosocomial infectious agents: a combination of extended-spectrum beta-lactamases and broad-spectrum beta-lactamases was identified in 62% of strains and the simultaneous presence of two different types of ESBLs was identified in 18% of strains.