Pseudomonas sp. (Strain 10–1B): A potential inoculum candidate for green and sustainable remediation

Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is a consequence of various industrial processes which destabilizes the ecosystem. Bioremediation by bacteria is a cost‐effective and environmentally safe solution for reducing or eliminating pollutants in soils. In the present study,...

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Bibliographic Details
Published in:Remediation (New York, N.Y.) N.Y.), 2017-06, Vol.27 (3), p.75-79
Main Authors: Bello‐Akinosho, Maryam, Adeleke, Rasheed, Thantsha, Mapitsi S., Maila, Mphekgo
Format: Article
Language:English
Subjects:
Bacteria
Biodegradation
Bioremediation
Communities
Cost engineering
Degradation
Deoxyribonucleic acid
DNA
Durability
Ecosystems
Ferredoxin
Fertility
Genomes
Hydroxylation
In vitro methods and tests
Indoleacetic acid
Inoculum
Nitrogen
Nucleotide sequence
Oil
Phosphate
Pollutants
Pollution
Pollution control
Polycyclic aromatic hydrocarbons
Proteins
Pseudomonas
Reductases
Remediation
Soil bacteria
Soil contamination
Soil fertility
Soil microorganisms
Soil pollution
Sustainability
Sustainable remediation
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Summary:Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is a consequence of various industrial processes which destabilizes the ecosystem. Bioremediation by bacteria is a cost‐effective and environmentally safe solution for reducing or eliminating pollutants in soils. In the present study, we artificially polluted agricultural soil with used automobile engine oil with a high PAH content and then isolated bacteria from the soil after 10 weeks. Pseudomonas sp. strain 10–1B was isolated from the bacterial community that endured this artificial pollution. We sequenced its genomic DNA on Illumina MiSeq sequencer and evaluated its ability to solubilize phosphate, fix atmospheric nitrogen, and produce indoleacetic acid, in vitro, to ascertain its potential for contribution to soil fertility. Its genome annotation predicted several dioxygenases, reductases, ferredoxin, and Rieske proteins important in the ring hydroxylation initiating PAH degradation. The strain was positive for the soil fertility attributes evaluated. Such combination of attributes is important for any potential bacterium partaking in sustainable bioremediation of PAH‐polluted soil.
ISSN:1051-5658
1520-6831
DOI:10.1002/rem.21521