A study of extracellular vesicles isolated from blood plasma conducted by low-voltage scanning electron microscopy

Extracellular vesicles secreted by cells represent an almost spherical membrane structures enriched with biological molecules of different types. The number and molecular composition of these structures depend on both the physiological state of an organism and underlying diseases. Despite extracellu...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue biology 2017-05, Vol.11 (3), p.181-190
Main Authors: Kondratov, K. A., Petrova, T. A., Mikhailovskii, V. Yu, Ivanova, A. N., Kostareva, A. A., Fedorov, A. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Blood
Cell Biology
Cell signaling
Electron microscopes
Electron microscopy
Extracellular vesicles
Life Sciences
Morphology
Physical characteristics
Potassium channels (voltage-gated)
Scanning electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extracellular vesicles secreted by cells represent an almost spherical membrane structures enriched with biological molecules of different types. The number and molecular composition of these structures depend on both the physiological state of an organism and underlying diseases. Despite extracellular vesicles playing an important role in intercellular communication and being potential biomarkers of pathological processes, the mechanisms of their formation, their functions, and their morphological characteristics are poorly studied. Low-voltage scanning electron microscopy is a promising method for studying extracellular vesicles, since it does not need a layer of conductive covering and, consequently, permits morphological details of studied objects to be vizualized at a high resolution in a nanometer range. The results of investigation of the morphology and sizes of objects in blood-plasma fractions by low-voltage scanning electron microscopy are presented in this study.
ISSN:1990-519X
1990-5203
DOI:10.1134/S1990519X17030051