Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy

Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, top-down MS analysis of high molecular weight (MW) proteins remains challenging mainly due to t...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-05, Vol.89 (10), p.5467-5475
Main Authors: Cai, Wenxuan, Tucholski, Trisha, Chen, Bifan, Alpert, Andrew J, McIlwain, Sean, Kohmoto, Takushi, Jin, Song, Ge, Ying
Format: Article
Language:English
Subjects:
Chemistry
Chromatography
Fractionation
gel chromatography
Genetic diversity
genetic variation
High resolution
Internet
Mass spectrometry
Mass spectroscopy
Molecular weight
Post-translation
post-translational modification
Protein purification
Proteins
proteome
Proteomics
reversed-phase liquid chromatography
Separation
Signal to noise ratio
Size exclusion chromatography
Translation
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