Umbelliferone stimulated melanogenesis and increased glutathione level in B16F10 cells

Umbelliferone (7-hydroxycoumarin) treatment caused an increase in melanin content in B16F10 melanoma cells in a dose-dependent manner, without causing toxicity. The increase in melanin content was correlated with increases in the activity of tyrosinase, the rate-limiting enzyme in melanin synthesis,...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology and environmental health sciences 2017-06, Vol.9 (2), p.152-160
Main Authors: Lee, Yunjung, Ku, Bonhee, Kim, Dongsoo, Choi, Eun-Mi
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Disorders
Environmental Health
Formulas (mathematics)
Glutathione
JNK protein
Kinases
MAP kinase
Melanin
Melanocyte-stimulating hormone
Melanoma
Microphthalmia-associated transcription factor
Original Article
Oxidation
Pharmacology/Toxicology
Proteins
Skin
Tanning
Toxicity
Tyrosinase
Tyrosinase-related protein 1
Ultraviolet radiation
Umbelliferone
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Umbelliferone (7-hydroxycoumarin) treatment caused an increase in melanin content in B16F10 melanoma cells in a dose-dependent manner, without causing toxicity. The increase in melanin content was correlated with increases in the activity of tyrosinase, the rate-limiting enzyme in melanin synthesis, and the expressions of melanogenic proteins, including tyrosinase, tyrosinase-related protein 1, and microphthalmia-associated transcription factor, the master transcriptional regulator for melanogenesis. Unlike α-melanocyte-stimulating hormone, umbelliferone did not cause melanogenesis-associated oxidation and depletion of glutathione. Conversely, umbelliferone treatment resulted in a significant and dose-dependent increase in glutathione. Umbelliferone caused activation of JNK, p38 MAPK, and GSK3β in a dose- dependent manner, suggesting possible involvement of those protein kinases in umbelliferone-induced stimulations of melanogenesis and antioxidant system. Our results suggest that umbelliferone stimulates both melanogenesis and antioxidant defense, providing more effective protection against UV-induced photodamage. They also imply possible applications of umbelliferone in self-tanning and treatment of skin disorders related with melanin deficiency.
ISSN:2005-9752
2233-7784
DOI:10.1007/s13530-017-0316-2