Enhanced dissipation of DEHP in soil and simultaneously reduced bioaccumulation of DEHP in vegetable using bioaugmentation with exogenous bacteria

• Published in: Biology and fertility of soils 2017-08, Vol.53 (6), p.663-675
• Main Authors: Zhao, Hai-Ming, Hu, Rui-Wen, Huang, He-Biao, Wen, Hong-Fei, Du, Huan, Li, Yan-Wen, Li, Hui, Cai, Quan-Ying, Mo, Ce-Hui, Liu, Jie-Sheng, Wong, Ming-Hung
• Format: Article
• Language: English
• Subjects: Agriculture; Bacteria; Bioaccumulation; Biodegradation; Biomedical and Life Sciences; Degradation; Dissipation; Food chains; Food safety; Hexanol; Hydrolysis; Hydroxylation; Inoculation; Landfills; Life Sciences; Original Paper; Phthalates; Plastics; Protocatechuic acid; Response surface methodology; Rhizosphere; Shoots; Soil contamination; Soil microorganisms; Soil pollution; Soil Science & Conservation; Soils; Vegetables; Waste disposal sites
• ISSN: 0178-2762; 1432-0789
• DOI: 10.1007/s00374-017-1208-y

The widely used plastic film containing di(2-ethylhexyl) phthalate (DEHP) in agriculture has caused serious soil pollution and poses risks to human health through the food chain. An effective DEHP degradation bacteria, Microbacterium sp. J-1, was newly isolated from landfill soil. Response surface methodology was successfully employed for optimization resulting in 96% degradation of DEHP (200 mg L −1 ) within 5 days. This strain degraded DEHP by hydrolysis of the ester bond and hydroxylation of the aromatic ring to form 2-ethyl hexanol, mono-(2-ethylhexyl) phthalate, phthalate acid, and protocatechuic acid, and subsequently transformed these compounds with a maximum specific degradation rate ( q max ), half-saturation constant ( K s ), and inhibition constant ( K i ) of 1.46 day −1 , 180.2 mg L −1 , and 332.8 mg L −1 , respectively. Bioaugmentation of DEHP-contaminated soils with the strain J-1 greatly enhanced the DEHP dissipation rate (~88%). Moreover, this strain could efficiently colonize the rhizosphere soil of inoculated vegetables and further enhanced DEHP degradation (~97%), leading to a significant decrease (>70%) in DEHP accumulation in shoots and roots of the inoculated vegetables compared to uninoculated vegetables. The results highlighted the roles of the inoculated exogenous bacteria in simultaneously bioremediating contaminated soils and reducing bioaccumulation of DEHP in the edible part of the vegetable for food safety.