Applicability of plant-based products in the treatment of Trypanosoma cruzi and Trypanosoma brucei infections: a systematic review of preclinical in vivo evidence

• Published in: Parasitology 2017-09, Vol.144 (10), p.1275-1287
• Main Authors: PEREIRA, RODRIGO M., GRECO, GLÍCIA M. Z., MOREIRA, ANDREIA M., CHAGAS, PABLO F., CALDAS, IVO S., GONÇALVES, REGGIANI V., NOVAES, RÔMULO D.
• Format: Article
• Language: English
• Subjects: African trypanosomiasis; Animal models; Animals; Bias; Biocompatibility; Biotechnology; Blood & organ donations; Cardiomyopathy; Cattle; Chagas disease; Chagas Disease - drug therapy; Chemotherapy; Drug Evaluation, Preclinical; Drugs; Flowers & plants; Indication; Infections; Medical research; Neglected Diseases - drug therapy; Parasitic diseases; Phytochemicals; Plant diseases; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant species; Plants; Plants (botany); Poverty; Product safety; Protozoa; Science; Studies; Toxicity; Trends; Tropical diseases; Trypanocidal Agents - pharmacology; Trypanocidal Agents - therapeutic use; Trypanosoma brucei brucei - drug effects; Trypanosoma cruzi - drug effects; Trypanosomiasis, African - drug therapy; Trypanosomiasis, Bovine - drug therapy; Vector-borne diseases
• ISSN: 0031-1820; 1469-8161
• DOI: 10.1017/S0031182017000634

Chagas disease and sleeping sickness are neglected tropical diseases closely related to poverty, for which the development of plant-derived treatments has not been a promising prospect. Thus, we systematicaly review the preclinical in vivo evidence on the applicability of plant-based products in the treatment of Trypanosoma cruzi and Trypanosoma brucei infections. Characteristics such as disease models, treatments, toxicological safety and methodological bias were analysed. We recovered 66 full text articles from 16 countries investigating 91 plant species. The disease models and treatments were highly variable. Most studies used native (n = 36, 54·54%) or exotic (n = 30, 45·46%) plants with ethnodirected indication (n = 45, 68·18%) for trypanosomiasis treatment. Complete phytochemical screening and toxicity assays were reported in only 15 (22·73%) and 32 (48·49%) studies, respectively. The currently available preclinical evidence is at high risk of bias. The absence of or incomplete characterization of animal models, treatment protocols, and phytochemical/toxicity analyses impaired the internal validity of the individual studies. Contradictory results of a same plant species compromise the external validity of the evidence, making it difficult determine the effectiveness, safety and biotechnological potential of plant-derived products in the development of new anti-infective agents to treat T. cruzi and T. brucei infections.