Chemoprevention by Probiotics During 1,2-Dimethylhydrazine-Induced Colon Carcinogenesis in Rats

Background Probiotics are believed to have properties that lower the risk of colon cancer. However, the mechanisms by which they exert their beneficial effects are relatively unknown. Aim To assess the impact of probiotics in preventing induction of colon carcinogenesis in rats. Methods The rats wer...

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Bibliographic Details
Published in:Digestive diseases and sciences 2018-04, Vol.63 (4), p.900-909
Main Authors: Walia, Sohini, Kamal, Rozy, Dhawan, D. K., Kanwar, S. S.
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Cancer
Carcinogenesis
Colon
Colon cancer
Colorectal cancer
Gastroenterology
Glutathione transferase
Health aspects
Hepatology
Medicine
Medicine & Public Health
Oncology
Original Article
Oxidative stress
Prevention
Probiotics
Proteins
Rodents
Superoxide
Transplant Surgery
Tumor proteins
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Summary:Background Probiotics are believed to have properties that lower the risk of colon cancer. However, the mechanisms by which they exert their beneficial effects are relatively unknown. Aim To assess the impact of probiotics in preventing induction of colon carcinogenesis in rats. Methods The rats were divided into six groups viz., normal control, Lactobacillus plantarum (AdF10)-treated, Lactobacillus rhamnosus GG (LGG)-treated, 1,2-dimethylhydrazine (DMH)-treated, L . plantarum (AdF10) + DMH-treated and L . rhamnosus GG (LGG) + DMH-treated. Both the probiotics were supplemented daily at a dose of 2 × 10 10 cells per day. DMH at a dose of 30 mg/kg body weight was administered subcutaneously twice a week for the first 4 weeks and then once every week for a duration of 16 weeks. Glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and catalase as protein expression of genes involved in apoptosis were assessed during DMH-induced colon carcinogenesis in rats. Results DMH treatment decreased the activity of GSH, GPx, GST, SOD and catalase. However, AdF10 and LGG supplementation to DMH-treated rats significantly increased the activity of these enzymes. Further, DMH treatment revealed alterations in the protein expressions of various genes involved in the p53-mediated apoptotic pathway such as p53, p21, Bcl-2, Bax, caspase-9 and caspase-3, which, however, were shifted towards normal control levels upon simultaneous supplementation with probiotics. Conclusion The present study suggests that probiotics can provide protection against oxidative stress and apoptotic-related protein disregulation during experimentally induced colon carcinogenesis.
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-018-4949-z