Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model

Two major hurdles in nanomedicine are the limited strategies for synthesizing stealth nanoparticles and the poor efficacy of the nanoparticles in translocating across the blood brain barrier (BBB). Here we examined the uptake and transcytosis of iron oxide nanoparticles (IONPs) grafted with biomimet...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials science 2018-02, Vol.6 (2), p.314-323
Main Authors: Ivask, Angela, Pilkington, Emily H, Blin, Thomas, Käkinen, Aleksandr, Vija, Heiki, Visnapuu, Meeri, Quinn, John F, Whittaker, Michael R, Qiao, Ruirui, Davis, Thomas P, Ke, Pu Chun, Voelcker, Nicolas H
Format: Article
Language:English
Subjects:
Biomimetic Materials - chemistry
Biomimetics
Blood-brain barrier
Blood-Brain Barrier - metabolism
Brain
Cells, Cultured
Ferric Compounds - chemistry
Grafting
Humans
Hyperspectral imaging
Iron oxides
Magnetite Nanoparticles - adverse effects
Magnetite Nanoparticles - chemistry
Nanoparticles
Phosphorylcholine
Phosphorylcholine - chemistry
Polyethylene Glycols - chemistry
Quantitative analysis
Transcytosis
X ray fluorescence analysis
X ray reflection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two major hurdles in nanomedicine are the limited strategies for synthesizing stealth nanoparticles and the poor efficacy of the nanoparticles in translocating across the blood brain barrier (BBB). Here we examined the uptake and transcytosis of iron oxide nanoparticles (IONPs) grafted with biomimetic phosphorylcholine (PC) brushes in an in vitro BBB model system, and compared them with bare, PEG or PC-PEG mixture grafted IONPs. Hyperspectral imaging indicated IONP co-localization with cells. Quantitative analysis with total reflection X-ray fluorescence spectrometry showed that after 24 h, 78% of PC grafted, 68-69% of PEG or PC-PEG grafted, and 30% of bare IONPs were taken up by the BBB. Transcytosis of IONPs was time-dependent and after 24 h, 16-17% of PC or PC-PEG mixture grafted IONPs had passed the BBB model, significantly more than PEG grafted or bare IONPs. These findings point out that grafting of IONPs with PC is a viable strategy for improving the uptake and transcytosis of nanoparticles.
ISSN:2047-4830
2047-4849
DOI:10.1039/c7bm01012e