The effects of ozone application on genotoxic damage and wound healing in bisphosphonate-applied human gingival fibroblast cells

Objectives Medication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic...

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Bibliographic Details
Published in:Clinical oral investigations 2018-03, Vol.22 (2), p.867-873
Main Authors: Akdeniz, Sıdıka Sinem, Beyler, E., Korkmaz, Y., Yurtcu, E., Ates, U., Araz, K., Sahin, F. I., Torun, O. Y.
Format: Article
Language:English
Subjects:
Alendronic acid
Bisphosphonates
Cell culture
Comet assay
Cytotoxicity
Dentistry
Deoxyribonucleic acid
DNA
Epithelium
Fibroblasts
Genotoxicity
Gingiva
Medicine
Original Article
Osteonecrosis
Ozone
Pamidronic acid
Plasma
Wound healing
Zoledronic acid
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Summary:Objectives Medication-related osteonecrosis of the jaws (MRONJ) is an extremely therapy-resistant disease involving the jaws especially following bisphosphonate treatment. Bisphosphonates accumulate in bone in concentrations sufficient to be directly toxic to the oral epithelium. Current therapeutic options are inadequate for the prevention and treatment of MRONJ. The aim of this study was to investigate effects of ozone gas plasma therapy on wound healing in bisphosphonate-applied human fibroblasts. Material and methods Human primary gingival fibroblasts were cultured. Cytotoxic concentrations (IC50) of bisphosphonates (pamidronate (PAM), alendronate (ALN), and zoledronate (ZOL)) were determined by MTT test. A 60 μg/μl for 30 s of ozone gas plasma application was performed to all experimental culture flasks after drug treatment at 24-h intervals as 3 s/cm 2 . Genotoxic damages were evaluated by comet assay and wound healing was determined by in vitro scratch assay. Results PAM, ALN, and ZOL applications caused genotoxic damage on primary human gingival fibroblast DNA. Ozone gas plasma therapy significantly decreased the genotoxic damage ( p  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-017-2163-6