Chemometrically assisted optimization and validation of a new HPLC method for the determination of paliperidone in pharmaceuticals

Paliperidone is an antipsychotic drug, which is used for the acute and maintenance treatment of schizophrenia. In this study, a new method was developed for the determination of Paliperidone in its extended-release tablets. Face-centered central composite design was applied for optimization of the m...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2018-02, Vol.41 (3), p.129-134
Main Authors: Atila Karaca, Sakine, Yeniceli Uğur, Duygu
Format: Article
Language:English
Subjects:
Acetonitrile
Antipsychotics
Buffers
Bupropion
Calibration
Design optimization
Drugs
Experimental design
Flow velocity
High-performance liquid chromatography
high-performance liquid chromatography (HPLC)
Linearity
Liquid chromatography
Mental disorders
paliperidone
pH effects
pharmaceuticals
Schizophrenia
Tablets
validation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Paliperidone is an antipsychotic drug, which is used for the acute and maintenance treatment of schizophrenia. In this study, a new method was developed for the determination of Paliperidone in its extended-release tablets. Face-centered central composite design was applied for optimization of the method. Factors were decided as acetonitrile content, pH of the mobile phase and buffer concentration through preliminary studies. Optimal flow rate (1 mL/min), column temperature (35°C) and internal standard (Bupropion) were also determined during preliminary studies. Retention factors and tailing factors of Paliperidone and Bupropion were selected as responses. Derringer's desirability function was applied for simultaneously optimization of these four responses. Optimal conditions were predicted as phosphate buffer (pH:3, 23 mM): acetonitrile (76:24, v:v). Developed method was validated in terms of linearity, detection and quantification limits, accuracy, precision, specificity and robustness. Method was found linear in the concentration range of 0.125-100 µg/mL. Mean equation of the calibration curve was y = 0.0807 x - 0.0102 (R 2  = 0.9999). Accuracy and precision of the method was evaluated with recovery values (98-102%) and relative standard deviation values (
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2018.1426599