Evaluation of PCR markers for Phytophthora infestans mating type determination

Late blight is a devastating potato disease caused by Phytophthora infestans . This organism can reproduce asexually and sexually between the strains of two mating types named A1 and A2. Mating type is an important strain characteristic affecting the pathogen’s population structure. We validate diff...

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Bibliographic Details
Published in:European journal of plant pathology 2018-09, Vol.152 (1), p.33-44
Main Authors: Brylińska, Marta, Sobkowiak, Sylwester, Stefańczyk, Emil, Śliwka, Jadwiga
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Blight
Correlation analysis
Ecology
Genotypes
Late blight
Life Sciences
Local population
Markers
Mating types
Pathogens
Phytophthora infestans
Plant Pathology
Plant Sciences
Population structure
Potatoes
Weaning
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Summary:Late blight is a devastating potato disease caused by Phytophthora infestans . This organism can reproduce asexually and sexually between the strains of two mating types named A1 and A2. Mating type is an important strain characteristic affecting the pathogen’s population structure. We validate different PCR markers for P. infestans mating type determination by comparison of the results obtained with the markers (W16, S1, PHYB) with the pairing test results for 26 isolates collected worldwide and for a group of 146 Polish isolates. This study identifies an interesting feature of the isolates of genotype US-1. For all these A1 mating type isolates, the product specific for A2 isolates is amplified using the marker W16. Analysis of sequences of W16 PCR products indicates high similarity of the US-1 isolates with modern A2 mating type isolates. When US-1 isolates are excluded from analysis, 95 and 96% of isolates are correctly assigned by markers W16 and S1, respectively, when compared with the pairing test results. Marker PHYB produces 14% of discrepant results with the pairing test. Our results show that molecular markers can be useful tools for P. infestans mating type determination, but their application should be preceded by validation in each local population since their efficiency may vary depending on a pathogen’s genotype.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-018-1445-4