Effect of Secondary Equilibria on the Adsorption of Ibuprofen Enantiomers on a Chiral Stationary Phase with a Grafted Antibiotic Eremomycin

The chromatographic separation of ibuprofen enantiomers on a Nautilus-E chiral stationary phase with a grafted eremomycin antibiotic at high column loading is accompanied by distortion of the shape of chromatographic peaks. A model is proposed to explain this phenomenon. A number of factors are cons...

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Bibliographic Details
Published in:Russian Journal of Physical Chemistry A 2018-02, Vol.92 (2), p.361-367
Main Authors: Reshetova, E. N., Asnin, L. D., Kachmarsky, K.
Format: Article
Language:English
Subjects:
Adsorption
Antibiotics
Chemistry
Chemistry and Materials Science
Chromatography
Computer simulation
Enantiomers
Ibuprofen
Ionization
Mass transfer
Nonsteroidal anti-inflammatory drugs
Phase transitions
Physical Chemistry
Physical Chemistry of Surface Phenomena
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Summary:The chromatographic separation of ibuprofen enantiomers on a Nautilus-E chiral stationary phase with a grafted eremomycin antibiotic at high column loading is accompanied by distortion of the shape of chromatographic peaks. A model is proposed to explain this phenomenon. A number of factors are considered in the model: the ionization of ibuprofen in the mobile phase, the pH change in the mass transfer zone caused by ionization, and competitive adsorption involving buffer components. Simulations performed using this model within the theory of nonequilibrium chromatography allow the shape of chromatograms for large amounts of S - and R -ibuprofen samples to be predicted. The adsorption mechanism is found to be mainly ion-exchange. The contribution from the molecular adsorption of ibuprofen to the total retention is shown to be several percent.
ISSN:0036-0244
1531-863X
DOI:10.1134/S0036024418010223