Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns

In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitro neurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with...

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Bibliographic Details
Published in:Nano research 2018-05, Vol.11 (5), p.2532-2543
Main Authors: Kim, Seong-Min, Lee, Seyeong, Kim, Dongyoon, Kang, Dong-Hee, Yang, Kisuk, Cho, Seung-Woo, Lee, Jin Seok, Choi, Insung S., Kang, Kyungtae, Yoon, Myung-Han
Format: Article
Language:English
Subjects:
Adhesion
Atomic/Molecular Structure and Spectra
Biomedicine
Biotechnology
Cell adhesion
Cell adhesion & migration
Chemistry and Materials Science
Condensed Matter Physics
Cones
Coupling
Cytoskeleton
Elongation
Growth cones
Materials Science
Morphology
Nanotechnology
Proteins
Research Article
Silicon
Substrates
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Summary:In this study, we report that height-controlled vertically etched silicon nano-column arrays (vSNAs) induce strong growth cone-to-substrate coupling and accelerate In vitro neurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography-induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.
ISSN:1998-0124
1998-0000
DOI:10.1007/s12274-017-1878-7