Identification of polymorphisms associated with oleic acid percentage by pool-based genomewide association study in Japanese Black cattle

• Published in: Journal of animal science 2016-09, Vol.94, p.17-18
• Main Authors: Kawaguchi, F, Nakajima, A, Matsumoto, Y, Uemoto, Y, Fukushima, M, Yoshida, E, Iwamoto, E, Akiyama, T, Kohama, N, Kobayashi, E, Honda, T, Oyama, K, Mannen, H, Sasazaki, S
• Format: Article
• Language: English
• Subjects: Animals; Association analysis; Beef; Beef cattle; Bovidae; Cattle; Deoxyribonucleic acid; DNA; Fatty acid composition; Fatty acids; Genome-wide association studies; Genomes; Genotype & phenotype; Genotypes; Meat quality; Oleic acid; Palatability; Polymorphism; Single-nucleotide polymorphism
• ISSN: 0021-8812; 1525-3163

The monounsaturated fatty acid percentage, especially oleic acid, is 1 of the important traits for beef quality because it has positive effects on beef palatability and human health. In our previous study, we selected 100 animals with the highest and 100 animals with the lowest oleic acid percentage from 1,836 animals, and performed pool-based, genome-wide association study (GWAS), using Illumina BovineSNP50 Bead- Chip v2 to identify genomic regions associated with oleic acid percentage (ISAG conference 2014). GWAS analysis revealed 2 novel candidate regions for oleic acid percentage; 3 and 1 significant SNP on BTA9 and BTA14, respectively, were detected at 5% genomewide significance level. The objective of this study was to confirm these significant SNPs detected by pooling method and to validate the candidate regions for fatty acid composition. We genotyped the most significant SNPs in each region (Hapmap60557-rs29018515 on BTA9 and BTB-00554873 on BTA14), using 444 animals selected randomly from 1,836 animals. Association analysis showed that the both SNPs on BTA9 and BTA14 were significantly associated with oleic acid percentage (P = 0.0004 and p < 0.0001, respectively). In addition, Tukey-Kramer's honestly significant difference test revealed that T/T genotype in Hapmap60557-rs29018515 indicated 1.31 higher percentage of oleic acid than C/C genotype; A/A genotype in BTB-00554873 indicated 1.23 higher percentage of oleic acid than C/C (p < 0.05). Our results suggested that these SNPs would be the effective DNA markers for oleic acid percentage in Japanese Black cattle. Further investigation on gene polymorphisms in these candidate regions may lead to identification of the causative mutation for oleic acid percentage.