Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

[Display omitted] •An extracellular xylanase was produced from a novel engineered P. pastoris (rXynCf).•Biochemical characterization of rXynCf was performed.•Empty fruit bunch (EFB) was selected as a feedstock for bioconversion process.•rXynCf was applied in the hydrolysis process to produce sugars...

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Bibliographic Details
Published in:Process biochemistry (1991) 2018-02, Vol.65, p.130-135
Main Authors: Kim, Chan Kyum, Choi, Han Suk, Lee, Sang Jun, Lee, Ja Hyun, Lee, Ju Hun, Yoo, Hah Young, Han, Sung Ok, Kim, Seung Wook
Format: Article
Language:English
Subjects:
Biomass
Biorefineries
Biorefinery
Cellulomonas flavigena
Conversion
endo-1,4-β-Xylanase
Enzymatic hydrolysis
Hydrolysis
Lignocellulose
Molecular chains
Molecular weight
Pichia pastoris
Pretreatment
Refineries
Refining
Sugar
Xylanase
Xylose
Yeast
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Summary:[Display omitted] •An extracellular xylanase was produced from a novel engineered P. pastoris (rXynCf).•Biochemical characterization of rXynCf was performed.•Empty fruit bunch (EFB) was selected as a feedstock for bioconversion process.•rXynCf was applied in the hydrolysis process to produce sugars from EFB.•rXynCf achieved 95.5% sugars conversion compared with commercial xylanase. In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZαB and expressed in Pichia pastoris X-33. An extracellular endo-1,4-β-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38kDa molecular weight, 55°C optimum temperature and optimum pH of 6. Under the conditions, the Km and Vmax of rXynCf were 3.6±0.08mg/mL and 4505±52μmol/minmg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2017.11.001