Comparison of serological and virological findings from subgroup J avian leukosis virus-infected neoplastic and non-neoplastic flocks in Israel

Blood samples from nine broiler breeder flocks comprising five flocks clinically affected with myeloid leukosis tumours (ML+) and four tumour-free flocks from the same commercial background (ML-) were compared for avian leukosis virus subgroup J (ALV-J) serum antibodies by enzyme-linked immunosorben...

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Bibliographic Details
Published in:Avian pathology 2004-06, Vol.33 (3), p.281-287
Main Authors: Malkinson, M, Banet-Noach, C, Davidson, I, Fadly, A.M, Witter, R.L
Format: Article
Language:English
Subjects:
Animals
antibodies
Antibodies, Viral - blood
Antigens, Viral - blood
avian leukosis
Avian Leukosis - blood
Avian Leukosis - pathology
Avian Leukosis - virology
Avian leukosis virus
Birds
broiler chickens
Chickens
Comparative analysis
diagnostic techniques
disease diagnosis
DNA Primers
Enzyme-Linked Immunosorbent Assay
false-negative errors
Fibroblasts - virology
Fluorescent Antibody Technique
Israel
myeloid leukosis tumors
neoplasms
Pathology
Poultry Diseases - blood
Poultry Diseases - immunology
Poultry Diseases - virology
Reverse Transcriptase Polymerase Chain Reaction - methods
Tumors
vertebrate viruses
viral antigens
viremia
Viremia - veterinary
Viruses
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Summary:Blood samples from nine broiler breeder flocks comprising five flocks clinically affected with myeloid leukosis tumours (ML+) and four tumour-free flocks from the same commercial background (ML-) were compared for avian leukosis virus subgroup J (ALV-J) serum antibodies by enzyme-linked immunosorbent assay (ELISA), for antigenemia (group-specific antigen) by antigen-trapping ELISA and for viremia. Group-specific antigen was detected in the sera of 58.1% of ML+ birds and 46.4% of the ML- birds (P=not significant), while 45.5% of ML+ birds and 24.1% of the ML- birds had ALV-J antibodies (P = 0.065). In inoculated cell culture, 64.1% of the ML+ sera were viremic compared with 16.7% of the ML- sera (P = 0.001). Similar significant differences were found between the two groups of flocks when ALV-J viremia was detected by immunofluorescence using a monoclonal env antibody (P = 0.004), and for proviral DNA by polymerase chain reaction using two different sets of env-gene primers, H5-H7 (P = 0.001) and R5-F5 (P = 0.001). Using the primer pair R5-F5 the product size was approximately 1 kbp, while some heterogeneity in size among isolates was discernable. Our results indicate that a combination of diagnostic tests should be adopted in routine examination of tumour material in order to rule out false-negative findings.
ISSN:0307-9457
1465-3338
DOI:10.1080/0307945042000203380