Chronic Hyperinsulinemia Augments Deoxycorticosterone Acetate–Salt Hypertension

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 1994-01, Vol.23 (1 Suppl I), p.I-16-I-19
• Main Authors: Kitamura, Shin-Ichi, Seto, Shinji, Nagao, Shoichi, Matsuo, Kiyotaka, Akahoshi, Masazumi, Yano, Katsusuke
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Blood Glucose - metabolism; Blood Pressure - drug effects; Body Weight - drug effects; Cardiology. Vascular system; Clinical manifestations. Epidemiology. Investigative techniques. Etiology; Desoxycorticosterone; Epinephrine - blood; Hyperinsulinism - complications; Hyperinsulinism - physiopathology; Hypertension - chemically induced; Hypertension - complications; Hypertension - physiopathology; Insulin - blood; Insulin - pharmacology; Male; Medical sciences; Norepinephrine - blood; Rats; Rats, Sprague-Dawley; Renin - blood; Sodium - blood; Sodium - urine; Sodium, Dietary; Time Factors
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/01.HYP.23.1_Suppl.I16

To evaluate the effect of chronic hyperinsulinemia on blood pressure in salt-dependent hypertension, we infused insulin (1.0 IU/d, n=15) or saline (n=13) for 4 weeks into deoxycorticosterone acetate-salt hypertensive rats. The insulin infusion increased plasma insulin levels to 24±2 μU/mL, which was higher than in the saline-infused rats (9±1 μU/mL) but was still within the physiological range. Blood pressure was measured by the tail-cuff method twice a week, and daily sodium intake and urinary sodium excretion were calculated for 3 weeks. At week 4, arterial pressor responsiveness to norepinephrine, angiotensin II, and hexamethonium bromide was evaluated. After 14 days of chronic infusion, the insulin group showed a higher blood pressure than the saline group (on 21st day178±6 versus 156±5 mm Hg, P