Inhibition of the p53 Tumor Suppressor Gene Results in Growth of Human Aortic Vascular Smooth Muscle Cells: Potential Role of p53 in Regulation of Vascular Smooth Muscle Cell Growth

Loss of activity of the p53 tumor suppressor gene product has been postulated in the pathogenesis of human restenosis. Although the antioncogenes p53 and retinoblastoma (Rb) susceptibility gene have been reported to play a pivotal role in cell cycle progression in various cells, the role of p53 and...

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Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 1999-08, Vol.34 (2), p.192-200
Main Authors: Aoki, Motokuni, Morishita, Ryuichi, Matsushita, Hidetsugu, Hayashi, Shin-ichiro, Nakagami, Hironori, Yamamoto, Kei, Moriguchi, Atsushi, Kaneda, Yasufumi, Higaki, Jitsuo, Ogihara, Toshio
Format: Article
Language:English
Subjects:
Analysis of Variance
Aorta - cytology
Apoptosis
Arteriosclerosis - pathology
Atherosclerosis (general aspects, experimental research)
Base Sequence
Biological and medical sciences
Blood and lymphatic vessels
Blotting, Western
Cardiology. Vascular system
Cell Count
Cell Cycle
Cells, Cultured
Culture Media
Data Interpretation, Statistical
DNA - biosynthesis
DNA Fragmentation
Flow Cytometry
Genes, p53 - genetics
Genes, p53 - physiology
Genes, Retinoblastoma - genetics
Genes, Retinoblastoma - physiology
Humans
Medical sciences
Molecular Sequence Data
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Oligonucleotides, Antisense - genetics
Oligonucleotides, Antisense - pharmacology
Thrombomodulin - analysis
Transfection - genetics
Transfection - methods
Tubulin - analysis
Citations: Items that this one cites
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Summary:Loss of activity of the p53 tumor suppressor gene product has been postulated in the pathogenesis of human restenosis. Although the antioncogenes p53 and retinoblastoma (Rb) susceptibility gene have been reported to play a pivotal role in cell cycle progression in various cells, the role of p53 and Rb in the growth of human vascular smooth muscle cells (VSMC) has not yet been clarified. We used antisense strategy against p53 and Rb genes by the viral envelope-liposomal method. Transfection of antisense p53 oligodeoxynucleotides (ODN) alone resulted in an increase in DNA synthesis compared with control (P
ISSN:0194-911X
1524-4563
DOI:10.1161/01.HYP.34.2.192