Cometabolism of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene by Pseudomonas acidovorans M3GY grown on biphenyl

1,1-Dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), a toxic breakdown product of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), has traditionally been viewed as a dead-end metabolite: there are no published reports detailing enzymatic ring fission of DDE by bacteria in either soil or pure cult...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1998-06, Vol.64 (6), p.2141-2146
Main Authors: Hay, A.G. (University of California, Riverside, CA.), Focht, D.D
Format: Article
Language:English
Subjects:
Bacteria
BIFENIL
Biodegradable materials
Biodegradation, Environmental
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
BIPHENYL
Biphenyl Compounds - metabolism
BIPHENYLE
DDT - metabolism
Dichlorodiphenyl Dichloroethylene - metabolism
Environmental Pollutants - metabolism
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Insecticides - metabolism
METABOLISM
METABOLISME
METABOLISMO
Microbiology
Mission oriented research
Physiology and Biotechnology
Physiology and metabolism
Pollutants
PSEUDOMONAS
Pseudomonas - growth & development
Pseudomonas - metabolism
Spectroscopy, Fourier Transform Infrared
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Summary:1,1-Dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), a toxic breakdown product of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT), has traditionally been viewed as a dead-end metabolite: there are no published reports detailing enzymatic ring fission of DDE by bacteria in either soil or pure culture. In this study, we investigated the ability of Pseudomonas acidovorans M3GY to transform DDE and its unchlorinated analog, 1,1-diphenylethylene (DPE). While strain M3GY could grow on DPE, cells grown on DPE as a sole carbon source could not degrade DDE. Cells grown on biphenyl, however, did degrade DDE. Mass balance analysis of [14C]DDE showed transformation of more than 40% of the recoverable radioactivity. Nine chlorinated metabolites produced from DDE were identified by gas chromatography-mass spectrometry-Fourier-transform infrared spectrometry (GC-MS-FTIR) from cultures grown on biphenyl. Recovery of these metabolites demonstrates that biphenyl-grown cells degrade DDE through a meta-fission pathway. This study provides a possible model for biodegradation of DDE in soil by biphenyl-utilizing bacteria
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.64.6.2141-2146.1998