Target switching catalytic hairpin assembly and gold nanoparticle colorimetric for EGFR mutant detection

•We carried out isothermal, label-free, enzyme-free, ultrasensitive EGFR mutant DNA sensing using TSCHA and AuNP colorimetry.•It is possible to detect the target DNA at very low concentrations (7.7 fM).•EGFR mutant is detected in the presence of wild-type DNA, as little as 1%.•Our proposed method (T...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-05, Vol.261, p.497-504
Main Authors: Park, Chanho, Song, Youngjin, Jang, Kuewhan, Choi, Chang-Hwan, Na, Sungsoo
Format: Article
Language:English
Subjects:
Assembly
Catalysis
Catalytic hairpin assembly
Circulation tumor DNA
Colorimetric
Colorimetry
Deoxyribonucleic acid
DNA
DNA detection
Gold
High sensitivity
Mutation
Nanoparticles
Selectivity
Sensitivity
Sensitivity analysis
Studies
Switching
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Summary:•We carried out isothermal, label-free, enzyme-free, ultrasensitive EGFR mutant DNA sensing using TSCHA and AuNP colorimetry.•It is possible to detect the target DNA at very low concentrations (7.7 fM).•EGFR mutant is detected in the presence of wild-type DNA, as little as 1%.•Our proposed method (TSCHA) can be utilized as a platform to detect mutant DNA in blood of cancer patients. The detection of circulating tumor DNAs (ctDNAs) with high sensitivity plays an important role in liquid biopsy diagnosis. For the detection of ctDNAs, we investigated the applicability of a two-ways CHA technique and found there were several problems such as sensitivity and selectivity. For this reason, we revised our technique to three-ways target switching catalytic hairpin assembly (TSCHA). Our target DNA is epidermal growth factor receptor (EGFR) mutation DNA. EGFR mutation DNA is very long DNA (84 mer) and it is hard to detect such a long DNA. However, with a TSCHA method, we can produce a short catalyst DNA (c-DNA) using long target DNA. After the catalytic reaction between DNAs, AuNPs aggregate and the detection solution become blue from red. We quantify the aggregation by observing UV–vis spectrum and can obtain LOD as low as 7.7 fM. Also the selectivity of the detection method is very high. Because of the high sensitivity, high selectivity, and simplicity, the TSCHA technique has great potential as a platform to detect mutant DNA in blood of cancer patients.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.01.183