Chara spp. exhibit highly heterogeneous light adaptation, calcite encrustation and epiphyton patterns in a marl lake

• Published in: Aquatic botany 2018-06, Vol.147, p.1-10
• Main Authors: Sviben, Sanja, Matoničkin Kepčija, Renata, Vidaković-Cifrek, Željka, Sertić Perić, Mirela, Kružić, Petar, Popijač, Aleksandar, Primc, Biserka
• Format: Article
• Language: English
• Subjects: Adaptation; Algae; Calcite; Calcium; Carotenoids; Chara globularis; Chara subspinosa; Chlorophyll; Chlorophyll a; Ciliates; Composition; Encrustation; Epiphytes; Gravimetry; Lakes; Light; Light adaptation; Marl; Micro-metazoa; Morphology; Organic chemistry; Organic matter; Photosynthesis; Photosynthetic pigments; Pigments; Plitvice lakes; Protozoa; Seaweeds; Species; Spectrophotometry; Thalli; Thallus; Water chemistry
• ISSN: 0304-3770; 1879-1522
• DOI: 10.1016/j.aquabot.2018.01.007

•Chara calcite encrustation decreases with water depth and is higher in C. subspinosa than in C. globularis.•Photosynthetic pigments differ between upper and lower thallus parts and between depths.•Pigment ratios increase with depth indicating specific light adaptations.•Excessive calcite encrustation negatively affects protozoans and micro-metazoans in epiphyton. The aim of this work was to determine the relationships between calcite encrustation, photosynthetic pigments and the epiphyton community of upper and lower thallus parts of Chara spp. from different depths of a marl lake (Lake Prošće, NP Plitvice Lakes, Croatia). Samples were taken from two Chara species, spread across three lake depths (Chara subspinosa at 1 and 5 m, and Chara globularis at 10 m), and analysed spectrophotometrically for photosynthetic pigment (chlorophyll a, chlorophyll b and total carotenoids) composition, and gravimetrically for organic matter and calcite encrustation contents within the macroalgal species. Epiphytic (i.e., epialgal) protozoa and micro-metazoa were determined on live material. The amounts of calcite deposits on Chara were two times higher at the depths of 1 and 5 m, compared to 10 m – most likely due to water chemistry (i.e., pH, temperature and calcium concentrations), light availability and photosynthesis patterns as well as Chara species morphology differences. Photosynthetic pigments exhibited complex patterns, differing both between upper and lower charophyte thallus parts, and among depths, likely reflecting adaptations of the macroalgae and epiphytes to light conditions. Protozoans and micro-metazoans exhibited various assemblages at different depths, with C. subspinosa supporting highest diversity (42 taxa at 5 m) and C. globularis exhibiting highest abundance (up to 9000 ind/gDW). Peritrich ciliates and rotifers preferred Chara thalli with less extensive calcite encrustations and more complex structure.