Genomic and transcriptomic analysis ofSaccharomyces cerevisiae isolates with focus in succinic acid production

Abstract Succinic acid is a platform chemical that plays an important role as precursor for the synthesis of many valuable bio-based chemicals. Its microbial production from renewable resources has seen great developments, specially exploring the use of yeasts to overcome the limitations of using ba...

Full description

Saved in:
Bibliographic Details
Published in:FEMS yeast research 2017-09, Vol.17 (6)
Main Authors: Franco-Duarte, Ricardo, Bessa, Daniela, Gonçalves, Filipa, Martins, Rosa, Silva-Ferreira, António César, Schuller, Dorit, Sampaio, Paula, Pais, Célia
Format: Article
Language:English
Subjects:
Acid production
Acids
Gene expression
Genomes
Single-nucleotide polymorphism
Succinic acid
Sustainable yield
Yeast
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Succinic acid is a platform chemical that plays an important role as precursor for the synthesis of many valuable bio-based chemicals. Its microbial production from renewable resources has seen great developments, specially exploring the use of yeasts to overcome the limitations of using bacteria. The objective of the present work was to screen for succinate-producing isolates, using a yeast collection with different origins and characteristics. Four strains were chosen, two as promising succinic acid producers, in comparison with two low producers. Genome of these isolates was analysed, and differences were found mainly in genesSDH1,SDH3,MDH1 and the transcription factorHAP4, regarding the number of single nucleotide polymorphisms and the gene copy-number profile. Real-time PCR was used to study gene expression of 10 selected genes involved in the metabolic pathway of succinic acid production. Results show that for the non-producing strain, higher expression of genesSDH1, SDH2, ADH1, ADH3, IDH1 andHAP4 was detected, together with lower expression ofADR1 transcription factor, in comparison with the best producer strain. This is the first study showing the capacity of natural yeast isolates to produce high amounts of succinic acid, together with the understanding of the key factors associated, giving clues for strain improvement. This is one of the first studies showing the capacity of natural yeast isolates to produce relevant amounts of succinic acid, giving clues for further strain improvement.
ISSN:1567-1356
1567-1364
DOI:10.1093/femsyr/fox057